Treg/Th17细胞失衡及相关细胞因子表达水平与再生障碍性贫血的关系分析

doi:10.3969/j.issn.1006-6233.2020.10.036

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摘要 目的: 探讨Treg/Th17细胞失衡及相关细胞因子表达水平与再生障碍性贫血(Aplastic anemia,AA)的关系。方法: 选取2016年12月至2018年6月期间合作医院收治的94例AA患者为研究对象,根据疾病严重程度不同分为轻型AA(mild AA,MAA)组41例和重型AA(severe AA,SAA)组53例,选取同期体检健康者50例为对照组。采用流式细胞术检测外周血Treg、Th17水平,采用酶联免疫吸附法检测血清白介素(Interleukin,IL)-10、IL-6和IL-17水平,并分析Treg/Th17细胞失衡及相关细胞因子与AA病情严重程度及预后的关系。结果: SAA组Treg细胞水平、Treg/Th17低于对照组和MAA组,Th17水平高于对照组和MAA组,差异均有统计学意义(P<0.05);SAA组IL-10水平低于对照组和MAA组,IL-6和IL-17水平高于对照组和MAA组,差异有统计学意义(P<0.05);Treg、Treg/Th17及IL-10水平与疾病严重程度呈负相关(r1= -0.604,r2= -0.661,r3= -0.611,P均<0.05),Th17、IL-6和IL-17与疾病严重程度呈正相关(r1=0.475,r2=0.486,r3=0.535,P<0.05);经多元Logitic回归模型进行分析,Treg和Treg/Th17是不良预后(急性复发或死亡)的保护性因素(P<0.05)。结论: AA患者机体存在Treg/Th17细胞失衡及相关细胞因子水平失衡,且Treg/Th17细胞失衡与AA病情严重程度和预后密切相关。

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	王蕊
	董彩丽
	赵云婷
	王艳

关键词 ： Treg/Th17, 细胞因子, 再生障碍性贫血, 预后, 相关性

Abstract：Objective: To explore the relevance between Treg/Th17 cell imbalance, expression levels of related cytokines and aplastic anemia (AA). Methods: 94 AA patients who were admitted to the cooperative hospital from December 2016 to June 2018 were enrolled. According to disease severity, they were categorized into mild AA (MAA) group (41 cases) and severe AA (severe AA) group (53 cases). 50 healthy people who underwent physical examination in the hospital during the same period were enrolled as control group. The levels of Treg and Th17 in peripheral blood were detected by flow cytometry. The levels of serum IL-10, IL-6 and IL-17 were detected by enzyme-linked immunosorbent assay (ELISA). The relevance between Treg/Th17 cell imbalance, related cytokines and AA severity, prognosis was analyzed. Results: Treg cell level and Treg/Th17 in SAA group were lower than those in control group and MAA group, while Th17 level was higher than that in control group and MAA group (P<0.05). The level of IL-10 in SAA group was lower than that in control group and MAA group, while levels of IL-6 and IL-17 were higher than those in control group and MAA group (P<0.05). The levels of Treg, Treg/Th17 and IL-10 were negatively correlated with disease severity (r1=-0.604, r2=-0.661, r3=-0.611, P<0.05). Th17, IL-6 and IL-17 were positively correlated with disease severity (r1=0.475, r2=0.486, r3=0.535, P<0.05). After multivariate Logitic regression model analysis, Treg and Treg/Th17 were protective factors influencing disease prognosis (acute recurrence or death) (P<0.05). Conclusion: There is an imbalance of Treg/Th17 cells and related cytokines in AA patients. The imbalance of Treg/Th17 cells is closely related to the severity and prognosis of AA.

Key words： Treg/Th17 Cytokine Aplastic anemia Prognosis Correlation

基金资助:河北省卫生和计划生育委员会科研基金项目,(编号:20190782)

通讯作者: 赵云婷

引用本文:

王蕊, 董彩丽, 赵云婷, 王艳. Treg/Th17细胞失衡及相关细胞因子表达水平与再生障碍性贫血的关系分析[J]. 河北医学, 2020, 26(10): 1738-1742.
WANG Rui, DONG Caili, ZHAO Yunting, et al. An Analysis of the Relevance Between Treg/Th17 Cell Imbalance, Expression Levels of Related Cytokines and Aplastic Anemia. HeBei Med, 2020, 26(10): 1738-1742.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2020.10.036 或 http://www.hbyxzzs.cn/CN/Y2020/V26/I10/1738

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