Abstract：Objective: To investigate the expressions of microRNA-335-5p (miR-335-5p) and activated transcription factor 2 (ATF2) in cervical cancer and their relationship with clinicopathological characteristics of cervical cancer patients. Methods: 72 patients with cervical cancer who underwent surgical treatment in our hospital from August 2017 to October 2019 were selected as the experimental group, 65 patients with uterine leiomyoma who underwent surgical treatment in the same period were selected as the control group, and cervical tissues of cervical cancer and uterine leiomyoma patients who underwent surgical resection were taken as the research samples. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of miR-335-5p, ATF2 mRNA and matrix metalloproteinase 2(MMP2) mRNA; immunohistochemistry was used to detect the positive expression rate of ATF2 and MMP2 protein; the relationships between the expression of miR-335-5p, ATF2 and the clinicopathological characteristics of cervical cancer were analyzed; the factors affecting cervical cancer were analyzed by logistic regression analysis; and the correlation of expression of mir-335-5p, Atf2 and MMP2 was analyzed. Results: The expression levels of miR-335-5p, ATF2 mRNA and MMP2 mRNA in the experimental group were significantly higher than those in the control group (P<0.05). The positive expression rate of ATF2 and MMP2 protein in the experimental group was significantly higher than that in the control group (P<0.05). The expression levels of miR-335-5p, ATF2 and MMP2 in cervical cancer tissues were correlated with FIGO stage, depth of invasion and lymph node metastasis (P<0.05). Logistic multivariate regression analysis showed that miR-335-5p, ATF2 and MMP2 were risk factors for cervical cancer. There was a positive correlation between the expression of miR-335-5p, Atf2 and MMP2 in cervical cancer (P<0.05). Conclusion: The expressions of miR-335-5p and ATF2 are high in cervical cancer tissues. Both of them are related to FIGO stage, depth of invasion and lymph node metastasis, and they may be biological indicators affecting the occurrence and development of cervical cancer.
冉伟, 廖萍, 刘兴兰, 马晓洁. miR-335-5p和转录因子ATF2在宫颈癌中的表达关系研究[J]. 河北医学, 2020, 26(10): 1617-1623.
RAN Wei, LIAO Ping, LIU Xinglan, et al. The Relevance Between the Expressions of MiR-335-5p and Transcription Factor ATF2 in Cervical Cancer. HeBei Med, 2020, 26(10): 1617-1623.
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2020, Vol. 26 
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