Abstract：Objective: To study the effect of VEGF regulated by vitamin D (VD) and/or VD receptor (VDR) on bronchopulmonary dysplasia (BPD) in neonatal rats. Methods: BDP neonatal rats were constructed and divided neonatal rats into control group and BDP group. Rat lung epithelial type Ⅱ cells (RLE-6TN) were cultured, and divided into VDR overexpression group (Le-VDR lentivirus infection group), VDR overexpression control group (Le-Ctrl group), VDR knockdown group (Sh-VDR infection group), VDR knockdown control group (Sh-Ctrl group), VD treatment alone group, VEGF-A silencing group (siVEGF-A group), and Le-VDR and siVEGF-A co-treatment group (Le-VDR + siVEGF-A group). The regulated effect of VD and VDR on lung cells was confirmed by CCK-8 detection, flow cytometry, wound healing detection and in vitro adhesion detection. VDR and VEGF-A mRNA were detected by RTqPCR, and VDR and VEGF-A protein were detected by Western blot. Results: Compared with the control group, the VD level and VDR expression in BPD neonatal rats were downregulated (P<0.01); overexpression of VDR in RLE-6TN cells increased the cell proliferation, migration, and adhesion ability (all P<0.01), and the apoptosis rate were decreased (P<0.01); however, the effect of knocking down VDR in cells was opposite. VD stimulation inhibited cell apoptosis and increased cell proliferation, migration and adhesion (P<0.05); VDR overexpression positively regulated vascular endothelial growth factor (VEGF)-A (P<0.05); Moreover, VEGF-A and VDR were co-localized in the lung tissue and cells of BPD rats; furthermore, the expression levels of VEGF-A in lung tissue of BPD neonatal rats were decreased (P<0.05). Conclusion: VD inhibits the development of BPD by up-regulating the expression of VEGF-A.
赵婷, 王彦梅, 王乐. 维生素D调节血管内皮生长因子在支气管肺发育不良新生大鼠中的作用及机制研究[J]. 河北医学, 2020, 26(10): 1591-1596.
ZHAO Ting, WANG Yanmei, WANG Le. The Role and Mechanism of Vitamin D in Regulating Vascular Endothelial Growth Factor in Neonatal Rats with Broncho Pulmonary Dysplasia. HeBei Med, 2020, 26(10): 1591-1596.
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2020, Vol. 26 
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