慢性鼻-鼻窦炎患者鼻息肉组织中CD69 RANTES的表达意义研究

doi:10.3969/j.issn.1006-6233.2019.10.023

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摘要目的: 探讨慢性鼻-鼻窦炎患者鼻息肉组织中CD69、RANTES的表达意义。方法: 198名慢性鼻-鼻窦炎患者,分为不伴鼻息肉组92例,伴鼻息肉组106例;同时本院87名没有慢性鼻-鼻窦炎的健康志愿者作为对照组;测定各组CD69、RANTES mRNA、蛋白以及IL-4、IL-10、IL-12、IFN-γ、TNF-α、hs-CRP水平。结果: 伴鼻息肉组CD69、RANTES mRNA、RANTES评分高于不伴鼻息肉组、对照组,不伴鼻息肉组CD69、RANTES mRNA、RANTES评分高于对照组,差异均有统计学意义(P<0.05);伴鼻息肉组IL-4、IL-10、IL-12、TNF-α、hs-CRP水平高于不伴鼻息肉组、对照组,IFN-γ低于不伴鼻息肉组、对照组;不伴鼻息肉组IL-4、IL-10、IL-12、TNF-α、hs-CRP水平高于对照组,IFN-γ低于对照组,差异均有统计学意义(P<0.05);CD69、RANTES mRNA与IL-4、IL-8、IL-10、TNF-α、hs-CRP正相关关系明显,与IFN-γ负相关关系明显,差异有统计学意义(P<0.05);高水平的TNF-α、CD69、RANTES mRNA均为慢性鼻-鼻窦炎患者有鼻息肉组织的危险因素(P<0.05);CD69、RANTES mRNA两者的AUC相近,皆小于TNF-α;CD69、RANTES mRNA诊断慢性鼻-鼻窦炎的灵敏度、特异度相近(χ²=1.219、1.695,P>0.05),皆小于TNF-α(P<0.05)。结论: 慢性鼻-鼻窦炎患者鼻息肉组织中CD69、RANTES水平升高,其引发一系列炎症因子失衡可能是慢性鼻-鼻窦炎的发病机制;高水平的CD69、RANTES均为慢性鼻-鼻窦炎患者有鼻息肉组织的危险因素。

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	陈雪梅
	易述军
	杨礼
	沈强

关键词 ：慢性鼻-鼻窦炎, 鼻息肉组织, CD69, RANTES

Abstract：Objective: To investigate the significance of CD69 and RANTES expression in nasal polyps of patients with chronic rhinosinusitis (CRS). Methods: 198 cases of CRS and 87 cases of health examinees (control group) in our hospital were selected. CRS patients were divided into non-nasal polyp group (n=92) and nasal polyp group (n=106). Then the mRNA and protein levels of CD69, RANTES, and levels of IL-4, IL-10, IL-12, IFN-γ, TNF-α, and hs-CRP were measured and compared between groups. Results: The mRNA and protein levels of CD69 and RANTES ranking in a descending order was nasal polyp group, non-nasal polyp group and control group (P<0.05).The levels of IL-4, IL-10, IL-12, TNF-α, and hs-CRP ranking in a descending order was nasal polyp group, non-nasal polyp group and control group (P<0.05).The IFN-γ levels ranking in an ascending order was nasal polyp group, non-nasal polyp group and control group (P<0.05). The mRNA levels of CD69 and RANTES were positively correlated with IL-4, IL-8, IL-10, TNF-α and hs-CRP, and negatively correlated with IFN-γ (P<0.05). High levels of TNF-α, CD69, and RANTES mRNA were risk factors for nasal polyps in CRS patients (P<0.05). The AUC, sensitivity and specificity of CD69 and RANTES mRNA in the diagnosis of CRS were similar (χ²=1.219, 1.695, P>0.05), which were less than TNF-α (P<0.05). Conclusion: Elevated levels of CD69 and RANTES in nasal polyps cause a series of inflammatory factors imbalance, which may be the pathogenesis of CRS; moreover, the high levels of CD69 and RANTES are risk factors for nasal polyps in CRS patients.

Key words： CRS Nasal polyp tissue CD69 RANTES

基金资助:重庆市自然科学基金项目,(编号:CSTC2016JJA0034)

通讯作者: 沈强

引用本文:

陈雪梅, 易述军, 杨礼, 沈强. 慢性鼻-鼻窦炎患者鼻息肉组织中CD69 RANTES的表达意义研究[J]. 河北医学, 2019, 25(10): 1674-1679.
CHEN Xuemei, YI Shujun, YANG Li, et al. Significance of CD69 and RANTES Expression in Nasal Polyps of Patients with Chronic Rhinosinusitis. HeBei Med, 2019, 25(10): 1674-1679.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2019.10.023 或 http://www.hbyxzzs.cn/CN/Y2019/V25/I10/1674

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