Abstract：Objective: To study the effect of TIMP-1 on the invasion, migration and secretion of VEGF in the villous trophoblast cells. Methods: Anoxic reoxygenation was used to treat HTR8/SVneo in the villous trophoblastic cells; the expression of TIMP-1 in cells was detected by fluorescence quantitative PCR and Western blot, transfection of TIMP-1 siRNA in HTR8/SVneo cells, after the anoxia and reoxygenation treatment, the expression of TIMP-1 in cells was detected by fluorescence quantitative PCR and Western blot; the effects of low TIMP-1 on the migration and invasion of anoxic reoxygenated HTR8/SVneo cells were detected in the Transwell compartment; Western blot assay was used to detect the level of MMP-9 protein, the content of VEGF, sFlt-1 and sEng in the supernatant of the culture liquid was detected by ELISA. Results: The level of TIMP-1 mRNA and protein in HTR8/SVneo cells after anoxic reoxygenation was higher than that of cells without anoxic reoxygenation (P<0.05). The transfection of TIMP-1 siRNA in cells can reduce the expression of TIMP-1 in the hypoxic and reoxygenation cells. The invasion and migration of HTR8/SVneo cells decreased after anoxic reoxygenation, the level of MMP-9 protein in the cells decreased, the cell secretion of VEGF decreased, and the number of sFlt-1 and sEng increased. Compared with the cells without anoxic reoxygenation, the difference was statistically significant (P<0.05). Down regulation of TIMP-1 can promote the invasion and migration of HTR8/SVneo cells under hypoxia and reoxygenation, promote the expression of MMP-9 in cells, Inducing cells to secrete VEGF, decrease the secretion of sFlt-1 and sEng in cells. Conclusion: Down regulation of TIMP-1 promoted the invasion and migration of villous trophoblastic cells under hypoxia and reoxygenation, and promoted the secretion of VEGF by cells.
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2018, Vol. 24 
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