Abstract:Objective: To ensure the quality of endoscopic biopsy pipeline to provide evidence through the biopsy channel of cleaning brush to wash after endoscopic examination and treatment times on the research quality of endoscopic cleaning, the cleaning brush to wash the number / endoscopic pipeline times. Methods: 150 endoscopic gastroscope, including (75) and (75) colonoscopy were randomly divided into 3 groups, cleaning brush application of steel - plastic brush head wash endoscopy and colonoscopy biopsy pipeline, respectively take brushing times 1 times, 3 times, 5 times of brushing, brushing each time for 5 second, brushing the water temperature at 35 DEG -38 deg. After scrubbing the endoscopic biopsy pipe, the pipe was sampled and then the biopsy pipe was thrown under the microscope to see the extent of the biofilm. Results:Cleaning brush steel - plastic brush head washed 3 times after endoscopic biopsy in the bacterial colonies, pipeline biofilm quantity, were significantly better than brushing 1 times, P<0.01. But there was no difference between the 5 times of scrubbing, p>0.05. Conclusion: the cleaning brush used for gastroscopy and colonoscopy biopsy is 5 times steel wire plastic brush, 35 seconds -38, and 3 times for the endoscopic biopsy tube. The cleaning brush of gastroscopy and colonoscope biopsy pipe used steel wire - plastic brush head, brushing time 5 seconds, water temperature 35 -38 C, and brushing endoscopic biopsy pipe 3 times.