has-miR-1-3p通过调控BDNF/TrkB信号通路抑制人滋养细胞增殖和侵袭

doi:10.3969/j.issn.1006-6233.2018.12.004

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摘要 目的: 探讨has-miR-1-3p对人滋养细胞HTR-8/SVneo增殖和侵袭的影响及其相关机制。方法: HTR-8/Svneo细胞分为两组,分别转染has-miR-1-3p模拟物作为实验组及无义miRNA为对照组,采用CCK8和Transwell实验分别检测对细胞增殖和侵袭能力的影响。通过在线分析网站预测has-miR-1-3p的靶基因,采用双荧光素酶报告实验和Western blot实验验证has-miR-1-3p对靶基因的调控。结果: 与对照组相比,has-miR-1-3p模拟物显著降低了HTR-8/Svneo细胞增殖和侵袭能力;双荧光素酶报告实验和Western blot实验结果表明has-miR-1-3p能够靶向抑制BDNF的表达,抑制TrkB的磷酸化;上调BDNF可降低has-miR-1-3p对HTR-8/Svneo细胞增殖和侵袭能力的抑制。结论: has-miR-1-3p通过调控BDNF/TrkB信号通路抑制人滋养细胞增殖和侵袭。

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	肖艳平
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关键词 ： has-miR-1-3p, 滋养细胞, 增殖, 侵袭, BDNF

Abstract：Objective: To investigate the role and mechanism of has-miR-1-3p in proliferation and invasion of human trophoblast cell line HTR-8/Svneo. Methods: HTR-8/Svneo cells was transfected with has-miR-1-3p mimics as experimental group, and nonsense miRNAs were used as the control group. CCK8 and Transwell assays were used to detect the effects of has-miR-1-3p on cell proliferation and invasion. The target genes of has-miR-1-3p were predicted by an online analysis website. The regulation of has-Mi-1-3p on target genes was verified by dual luciferase report and Western blot assays. Results: has-miR-1-3p mimics significantly decreased cell proliferation and invasion of HTR-8/Svneo cells compared to the control cells. Moreover, the dual luciferase reporter and Western blot assays indicated that has-miR-1-3p mimics could directing target BDNF, and the phosphorylation level of TrkB was also inhibited. Furthermore, up-regulation of BDNF significantly reversed the inhibitory effects of has-miR-1-3p mimics on proliferation and invasion in HTR-8/Svneo cells. Conclusion: has-miR-1-3p inhibits proliferation and invasion of human trophoblast cells via regulating BDNF/TrkB pathway.

Key words： has-miR-1-3p Trophoblast cells Proliferation Invasion BDNF

引用本文:

肖艳平,付久园,葛永梅,张金环. has-miR-1-3p通过调控BDNF/TrkB信号通路抑制人滋养细胞增殖和侵袭[J]. 河北医学, 2018, 24(12): 1948-1952.
XIAO Yanping, FU Jiuyuan, GE Yongmei, et al. has-miR-1-3p Inhibits Proliferation and Invasion of Human Trophoblast Cells Via Regulating BDNF/TrkB Pathway. HeBei Med, 2018, 24(12): 1948-1952.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2018.12.004 或 http://www.hbyxzzs.cn/CN/Y2018/V24/I12/1948

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