Abstract：Objective: To observe the changes of sensitive indexes in early diagnosis of intestinal mucosal injury and the effect on intestinal mucosal barrier function in rats with traumatic hemorrhagic shock. Methods: 50 adult male Sprague-Dawley rats were selected and randomly divided into control group (N=10), 1h shock group (N=10), 4h shock group (N=10), 8h shock group (N=10), 16h shock group (N=10). After the traumatic hemorrhagic shock model was established in rats, colorimetric assay was used to measure the DAO activity in rat plasma. The endotoxin and D-lactate levels in rat plasma were determined by spectrophotometry. Rat ileum tissue was harvested and the histopathological changes were observed. Venous blood and ileal tissue were collected at the same time points in the control group and the shock 1h group for detection and observation. The data were analyzed by SAS 9.4 professional statistical software. Measured data were expressed as mean±standard deviation. The comparison between groups was performed using one-way analysis of variance, and the comparisons between groups were made using the LSD test The shock group was compared with the control group using the SNK method. P<0.05 (both sides) was statistically significant. Results: With the extension of time of shock, the D- lactic acid, diamine oxidase and endotoxin content of each observation groups were gradually increased. And the differences between them were significant (P<0.05). In the control group, the intestinal epithelium was structurally intact and no villus was absent and the morphology was normal. In the observation group, the intestinal mucosal structure was intact and the intestinal villi were irregular in the 1h shock group; part of the intestinal epithelium was shed in the 4h shock group, with local edema and no obvious inflammatory cell infiltration; the epithelial shedding of the 8h group was increased, and the local edema was obvious. There was obvious inflammatory cell infiltration in the lamina propria; the epithelial tissue in the 16h group was severely shedding, and the edema was severe. The infiltration of inflammatory cells in the lamina propria was more obvious than that in the 8h group. Conclusion: In the early stage of traumatic hemorrhagic shock, intestinal barrier dysfunction occurs. Plasma D-lactate, DAO and endotoxin are sensitive indicators for the early diagnosis of intestinal mucosal injury, and their levels are positively correlated with the pathological changes of intestinal mucosal tissues.
刘海. 创伤失血性休克大鼠肠黏膜损伤早期诊断敏感指标的变化及意义[J]. 河北医学, 2018, 24(10): 1606-1609.
LIU Hai. Changes and Significance of Sensitive Indexes in Early Diagnosis of Intestinal Mucosal Injury after Traumatic Hemorrhagic Shock in rats. HeBei Med, 2018, 24(10): 1606-1609.
2018, Vol. 24 
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