不同剂量右美托咪定复合地塞米松对肺缺血再灌注大鼠模型炎症因子白三烯及组胺水平的影响

doi:10.3969/j.issn.1006-6233.2018.01.039

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摘要 目的: 探讨不同剂量右美托咪定复合地塞米松对肺缺血再灌注大鼠模型炎症因子白三烯及组胺水平的影响。方法: 选取SD大鼠50只,按随机数字表分为假手术组、模型组、右美托咪定高剂量组、右美托咪定中剂量组、右美托咪定低剂量组,每组10只。参照文献报道,建立肺缺血再灌注损伤模型。造模成功后,右美托咪定低、中、高剂量组大鼠给予右美托咪定1、5、10μg/kg负荷剂量,然后以1、5、10μg·kg^-1·h^-1持续输注1h;均给予地塞米松0.2mg/kg,气管内滴注,然后以3mg/kg静脉注射;模型组以及假手术组均给予等量的生理盐水静脉滴注,连续用药4周。观察并比较各组大鼠肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、组胺(HIS)以及白三烯B4(LTB4)水平。结果: 与模型组相比,右美托咪定高剂量组和右美托咪定中剂量组的TNF-α、IL-1β、HIS、LTB4水平均下降,差异具有统计学意义(P<0.05),右美托咪定高剂量组和右美托咪定中剂量组的TNF-α水平相比,差异无统计学意义(P>0.05)。结论: 右美托咪定复合地塞米松能够控制肺缺血再灌注大鼠模型的炎症水平,降低白三烯及组胺水平,但加大剂量后保护作用有限,未显示出显著的保护作用。

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	李冠军

关键词 ：右美托咪定, 地塞米松, 肺缺血再灌注, 炎症因子, 白三烯, 组胺

Abstract：Objective: To investigate the effects of different doses of dexmedetomidine combined with dexamethasone on the levels of inflammatory cytokines and histamine in rats with lung ischemia-reperfusion injury. Methods: 50 SD rats were randomly divided into four groups: sham operation group, model group, dexmedetomidine high dose group, dexmedetomidine group, dexmedetomidine low dose group, each group of 10 rats. According to the literatures, the model of lung ischemia-reperfusion injury was established. After the modeling, dexmedetomidine low, middle and high dose group rats were given dexmedetomidine 1, 5, 10μg/kg loading dose, then in 1, 5, 10μg·kg^-1·h^-1 were treated with continuous infusion of 1H; dexamethasone 0.2 mg/kg, intratracheal instillation, and then to 3 mg/kg intravenous injection; intravenous infusion of saline model group and sham operation group were given the same amount of medication for 4 weeks. The tumor necrosis factor α (TNF-α), interleukin -1 β (IL-1β), histamine (HIS) and leukotriene B4 (LTB4) levels were observed and compared in each group. Results: Compared with model group, dexmedetomidine group and high dose of dexmedetomidine in dose of TNF- α, IL-1β, HIS and LTB4 levels were decreased, the difference was statistically significant (P<0.05), TNF- α, IL-1β, HIS and LTB4 levels in dexmedetomidine high dose group and dexmedetomidine dose group, the difference was not statistically significant (P>0.05). Conclusion: Dexmedetomidine combined with dexamethasone can control lung ischemia reperfusion rat model of inflammation, reduce the leukotriene and histamine levels, but increase the dose limited protective effect, showed no significant protective effect.

Key words： Dexmedetomidine Dexamethasone Lung ischemia reperfusion Inflammatory factor Leukotrienes Histamine

基金资助:山东省卫生厅科研计划项目,(编号:2012ws1782)

引用本文:

李冠军. 不同剂量右美托咪定复合地塞米松对肺缺血再灌注大鼠模型炎症因子白三烯及组胺水平的影响[J]. 河北医学, 2018, 24(1): 145-152.
LI Guanjun. Effects of Different Doses of Dexmedetomidine combined with Dexamethasone on the Levels of Inflammatory Cytokines and Histamine in rats with Lung Ischemia-reperfusion Injury. HeBei Med, 2018, 24(1): 145-152.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2018.01.039 或 http://www.hbyxzzs.cn/CN/Y2018/V24/I1/145

[1] 宋兆孟,蒋琦亮,陈旭,等.右美托咪定对大鼠肺移植后肺缺血再灌注损伤的影响[J].上海医学,2016(2):112~115.
[2] 罗梓垠,项冰倩,宋冬,等.右美托咪定对小鼠肺缺血再灌注时JNK表达的影响[J].中华麻醉学杂志,2016,36(6):762~764.
[3] 朱宏伟,吴镜湘,蒋琦亮,等.右美托咪定对大鼠肺缺血再灌注损伤后诱导型一氧化氮合酶表达的影响[J].上海医学,2015(2):129~132.
[4] 朱洁芳,董铁立,ZhuJiefang,等.右美托咪定预处理和后处理对大鼠肺缺血再灌注所致急性肺损伤的影响[J].中华实验外科杂志,2016,33(6):1604~1607.
[5] 张伟,张卫,张加强.PI3K/Akt/HIF-1α信号通路在右美托咪定减轻大鼠肺缺血再灌注损伤中的作用[J].中国医院药学杂志,2016,36(21):1890~1893.
[6] 郭长满,陈丹,宋东,等.右美托咪定对小鼠肺缺血-再灌注损伤的保护作用[J].中国临床药理学与治疗学,2016,21(2):145~149.
[7] 吕兴华,冷玉芳,杨艳妮,等.右美托咪定对大鼠肺缺血再灌注时脂质过氧化反应的影响[J].中华麻醉学杂志,2016,36(4):508~510.
[8] 罗梓垠,郭长满,项冰倩,等.右美托咪定对肺缺血/再灌注损伤小鼠内质网应激相关分子Caspase-12表达的影响[J].中国应用生理学杂志,2016,32(2):164~168.
[9] 金海珍.右美托咪定对肺缺血再灌注损伤小鼠JNK表达的影响[D].温州医科大学,2016.
[10] 陈月,张加强,孟凡民.右美托咪定对肢体缺血再灌注诱发肺损伤的影响[J].中国医院药学杂志,2015,35(12):1113~1115.
[11] 徐雪,蔡劲松,戚翔,等.不同剂量右美托咪定对大鼠全脑缺血再灌注损伤脑保护作用的研究[J].现代中西医结合杂志,2015(7):688~690.
[12] 解春艳,李云峰,梁江水,等.右美托咪定对肠缺血再灌注肺损伤中自噬与凋亡的影响[J].中华结核和呼吸杂志,2015,38(10):761~764.
[13] 吴志林,褚淑娟,姚尚龙,等.不同剂量右美托咪定预处理对大鼠心肌缺血再灌注损伤以及炎症反应的影响[J].华中科技大学学报医学版,2015(4):445~447.
[14] 程中贵,孙杨,程楼英.右美托咪定预处理对止血带引起肢体缺血再灌注致肺损伤的保护作用[J].医学信息,2016,29(22):75~76.
[15] 戚翔,贾建丽,蔡劲松,等.不同剂量右美托咪定预处理对大鼠全脑缺血再灌注损伤炎性反应的影响[J].临床外科杂志,2015(11):852~854.
[16] 李新灵,雷钟,杜靖,等.不同剂量右美托咪定对大鼠肾缺血再灌注后心肌组织损伤的影响[J].医学研究生学报,2015(8):809~814.
[17] 徐刚,卢锡华.右美托咪定预处理对小鼠肺缺血-再灌注损伤时微小RNA-210表达的影响[J].中华实验外科杂志,2016,33(4):1008~1011.