2025年4月3日 星期四
首页        期刊介绍        编委会        投稿指南        期刊订阅        广告合作        联系我们        English
河北医学  2017, Vol. 23 Issue (8): 1236-1241    DOI: 10.3969/j.issn.1006-6233.2017.08.002
  论 著 本期目录 | 过刊浏览 | 高级检索 |
血浆MicroRNA-17-5p和microRNA-20水平与肺动脉高压预后的相关性研究
张岩, 王聪霞, 李永勤, 张春艳, 胡艳超, 李满祥
西安交通大学第二附属医院心血管内科,陕西 西安 710004
Correlation between Plasma Levels of MicroRNA-17-5p and microRNA-20 and Prognosis of Pulmonary Arterial Hypertension
ZHANG Yan, WANG Congxia, LI Yongqin, et al
The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi Xi'an 710004,China
全文: PDF (1873 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 目的: 探讨患者血浆MicroRNA-17-5p和microRNA-20水平与与肺动脉高压(pulmonary arterial hypertension,PAH)严重程度、预后的相关性。方法: 采用微阵列法筛选112例PAH患者和100例健康对照者的血浆总RNA,采用实时定量聚合酶链反应测定miRNA-17-5p和miRNA-20a含量,测量PAH患者血浆中miRNA-17-5p、miRNA-20a的浓度。结果: 在血浆miRNA浓度测定中,总共有58例miRNA血浆浓度在病例组和对照组中差异具有显著性。在PAH组中,miRNA-17-5p和miRNA-20a上调最为明显。ROC曲线分析显示血浆miRNA-17-5p、miRNA-20a的浓度与PAH患者两年生存率相关。Cox回归分析证实miRNA-17-5p和miRNA-20a水平是PAH生存率的重要预测因素。年龄、心脏指数、WHO分级、6min步行距离、疾病持续时间、红细胞分布宽度也可作为生存率的预测因子,加入这些协变量的多变量模型验证miRNA-17-5p和miRNA-20a水平是PAH两年生存率的独立预测因子。结论: 血浆中miRNA-17-5p和miRNA-20a水平的增加与PAH患者的不良预后有关。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
关键词 miRNA-17-5pmiRNA-20a肺动脉高压    
AbstractObjective: To investigate the correlation between the level of plasma MicroRNA-17-5p and microRNA-20 and the severity and prognosis of pulmonary arterial hypertension (PAH). Methods: A microarray screen was performed on total plasma RNA from 112 patients with PAH and 100 healthy control subjects Quantitative polymerase chain reaction commend increased microRNA-17-5p and microRNA-20a concentrations and was then used to measure microRNA-17-5p and microRNA-20a levels in cohort of patients with PAH's circulating microvesicles and blood cells. Results: Fifty-eight miRNAs showed differences in plasma concentration. MicroRNA-17-5p and microRNA-20a are the largest up-regulation in PAH. Receiver-operator-characteristic analysis showed both raw and normalized plasma microRNA-17-5p and microRNA-20a levels correlated with 2-year survival in patients with PAH. Cox regression analysis confirmed that miRNA-17-5p and miRNA-20a levels were important predictors of PAH survival. Age, baseline cardiac index, World Health Organization functional class, 6-minute walk distance, disease duration, and red cell distribution width also predicted survival. Entering these covariates in a multivariable model veried plasma microRNA-17-5p and microRNA-20a levels as independent predictoras of survival in PAH. Conclusion: Increased circulating microRNA-17-5p and microRNA-20a levels are associated with poor survival in PAH. 
Key wordsmiRNA-17-5p    miRNA-20a    Pulmonary arterial hypertension
    
通讯作者: 王聪霞   
引用本文:   
张岩, 王聪霞, 李永勤, 张春艳, 胡艳超, 李满祥. 血浆MicroRNA-17-5p和microRNA-20水平与肺动脉高压预后的相关性研究[J]. 河北医学, 2017, 23(8): 1236-1241.
ZHANG Yan, WANG Congxia, LI Yongqin, et al. Correlation between Plasma Levels of MicroRNA-17-5p and microRNA-20 and Prognosis of Pulmonary Arterial Hypertension. 河北医学, 2017, 23(8): 1236-1241.
链接本文:  
http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2017.08.002     或     http://www.hbyxzzs.cn/CN/Y2017/V23/I8/1236
冀ICP备2025106803号    冀公网安备13080202000786号
版权所有 © 2016 《河北医学》杂志社
本系统由北京玛格泰克科技发展有限公司设计开发