Abstract：Objective: To observe the effects of Rumex japonicus Houtt extract on transforming growth factor-β1 and its receptors in thrombocytopenic purpura mice model. Methods: 40 BALB/C mice were randomly divided into positive control group, model group, rumex japonicus houtt extract group and negative control group. All ITP mouse model were made by injected guinea pig-antimouse platelet serum (GP-APS) except negative control group. The expressions of TGF-β1 and their receptors TGF-βR in the peripheral blood of mice were detected by the real-time PCR and Western Blot, and differences of their expression levels were analyzed. Results: The expression of TGF-β1 in ITP model group was significantly higher than that in the negative control group (P<0.05), while the TGF-β mRNA and TGF-β protein expression in ITP model group were significantly lower than that in the negative control group (P<0.05). The expression of TGF-β1 in rumex japonicus houtt extract group was significantly lower than that in the ITP model group (P<0.05), while the TGF-β mRNA and TGF-β protein expression in rumex japonicus houtt extract group were significantly higher than that in the ITP model group (P<0.05). Conclusion: Rumex japonicus houtt extract can regulate the immune function of ITP by in fluencing the expression of transforming growth factor-β1 and its receptors.
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2017, Vol. 23 
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