Abstract：Objective: To investigate the effects of valproic acid ,one of histone deacetylase(HDAC) inhibitors, combinations with PTX, on the expression of vascular endothelial growth factor (VEGF) and Ang-2 of the lung cancer cell line A549 cell. Explore their significance of cell proliferation, apoptosis and metastasis on A549 cell. Methods: Set the A549 cell to four groups:control group,PTX group,VPA group and VPA combined with PTX group. Determine the mRNA and protein of VEGF and Ang-2 by quantitative PCR and Western Blot. Results: The PTX group,VPA group and VPA combined with PTX can downregulate the VEGF and Ang-2 in mRNA and protein level. The combination group of lowest level of these two genes and protein. The difference between the groups was statistically significant. Conclusion: HDAC inhibitor valproic acid may depend on its regulation of histone to inhibite cell proliferation , promote cell apoptosis of A549 cell and inhibit the angiogenesis by regulating the VEGF and Ang-2 expression.
朱翠敏, 高菲菲, 梁云微, 连相尧, 胡潺潺, 李青山. 丙戊酸钠协同紫杉醇对肺癌A549细胞株血管新生相关因子表达的影响[J]. 河北医学, 2017, 23(3): 363-365.
ZHU Cuimin GAO Feifei, LIANG Yunwei, et al. The Effects of VPA Combined with PTX on the Expression of Angiogenesis Related Factors in A549 Lung Cancer Cell Line. HeBei Med, 2017, 23(3): 363-365.
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2017, Vol. 23 
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