Abstract：Objective: To investigate the prevalence of physical state of HVP-16 DNA in cervical and cervical precancerous carcinoma. Methods: Multiplex PCR was adopted to detect the physical state of HPV in samples from 252 patients with cervical carcinoma, including 48 samples of cervical cancer, 204 cervical intraepithelial(CIN)(125 CIN I,46 CIN Ⅱ and 33 CIN Ⅲ)and 20 normal samples from the subjects with hysteromyoma undergoing hysterectomy ,respectively. Results: Not only patients with cervical cancer but also patients with precancerous were found to have integrated infection with HPV-16 and those with cervical cancer have the highest integration rate. With the elevating of the grades of CIN, the integrated infection rates of HVP-16 was significantly elevated, the difference was statistically significant (χ2=49.7359,12.6033, P<0.01). Conclusion: Among the patients with HPV-16 infection,the integrated state of HVP-16 is positively correlated with the severity of cervical lesions. Combined HVP typing test and detection of integrated viral contribute to predicting the prognosis of patients with cervical precancerous lesions and increasing the accuracy of screening cervical cancer on the basis of HPV DNA detection.
王霞. 宫颈癌及癌前病变中的HPV-16整合人宿主基因组的发生情况及其在宫颈癌筛查中的应用[J]. 河北医学, 2017, 23(11): 1768-1771.
WANG Xia. Integrated State of HPV-16 in Precancerous Tissues and CervicalCancer and Application in Cervical Cancer Screening. HeBei Med, 2017, 23(11): 1768-1771.
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2017, Vol. 23 
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