鼻咽癌组织中miR-140-3p AGT5表达变化及临床意义

doi:10.3969/j.issn.1006-6233.2025.02.021

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摘要 目的: 研究探讨鼻咽癌组织中微小RNA140-3p(miR-140-3p)、自噬相关基因5(AGT5)表达与病理参数及预后的关系。方法: 选取102例鼻咽癌患者为研究对象,采用荧光定量PCR技术检测患者鼻咽癌组织和癌旁组织中的miR-140-3p、AGT5的表达情况,对比癌组织和癌旁组织中miR-140-3p、AGT5表达水平及相关性。并分析鼻咽癌组织中miR-140-3p、AGT5表达水平与患者临床病理特征参数的关系,随访3年,统计对比不同miR-140-3p、AGT5表达患者预后差异,Cox回归分析患者预后影响因素。结果: 鼻咽癌组织中的miR-140-3p、AGT5表达水平低于癌旁组织(t=23.657、27.625,P=0.000、0.000)。鼻咽癌组织中miR-140-3p与AGT5表达呈显著正相关(r =0.614,P=0.000)。高分化、TNM分期为Ⅰ~Ⅱ期、无淋巴结转移患者中miR-140-3p、AGT5表达水平高于中低分化、TNM分期Ⅲ~Ⅳ期、有淋巴结转移患者(P<0.05)。患者3年总体生存率为75.49%(77/102)。miR-140-3p(≥0.97,n=49)、AGT5(≥1.46,n=48)高表达患者3年总体生存率为87.76%(43/49)、87.50%(42/48),高于miR-140-3p(<0.97,n=53)、AGT5(<1.46,n=54)低表达的64.15%(34/53)、64.81%(35/54),差异有统计学意义(Log-Rank 2=7.667、7.063,P=0.006、0.008)。分化程度低、TNM分期Ⅲ~Ⅳ期、淋巴结转移是鼻咽癌患者预后的独立危险因素,miR-140-3p、AGT5高表达是鼻咽癌患者预后的保护因素 (P<0.05)。结论: miR-140-3p、AGT5在鼻咽癌患者中呈现低表达,与鼻咽癌的发生、发展及预后生存率有密切关联,可能共同参与鼻咽癌的进展。

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关键词 ：鼻咽癌, 微小RNA140-3p, 自噬相关基因5, 病理特征, 预后

Abstract：Objective: To investigate the correlation between the expression of microRNA140-3p (miR-140-3p) and autophagy related gene 5 (AGT5) with pathological parameters and prognosis of nasopharyngeal carcinoma patients. Methods: A total of 102 nasopharyngeal carcinoma patients were selected as the research subjects. The expression of miR-140-3p and AGT5 in nasopharyngeal carcinoma tissues and adjacent tissues was detected by fluorescent quantitative polymerase chain reaction (qPCR) technology. The correlation of the expression levels of miR-140-3p and AGT5 in cancer tissues and adjacent tissues with pathological parameters and prognosis of nasopharyngeal carcinoma patients was assessed. After 3-year follow-up, the prognosis of patients with different expressions of miR-140-3 p and AGT5 was compared. Cox regression analysis was employed to analyze the factors affecting patient prognosis. Results: The expression levels of miR-140-3p and AGT5 in nasopharyngeal carcinoma tissues were significantly lower than those in adjacent tissues (t=23.657, 27.625, P=0.000, 0.000). There was a significant positive correlation between miR-140-3p and AGT5 expression in nasopharyngeal carcinoma tissues (r=0.614, P=0.000). The expression levels of miR-140-3p and AGT5 were significantly higher in patients with high differentiation, tumor-node-metastasis (TNM) stages Ⅰ~Ⅱ, and no lymph node metastasis than those with medium low differentiation, TNM stages Ⅲ~Ⅳ, and lymph node metastasis (P<0.05). The 3-year overall survival rate was 75.49% (77/102) among those patients. The 3-year overall survival rates of patients with high expression of miR-140-3p (≥0.97 ,n=49) and AGT5 (≥1.46, n=48) were significantly higher than those with low expression of miR-140-3p (＜0.97, n=53) and AGT5 (＜1.46, n=54)(87.76% [43/49] vs 64.15% [34/53], 87.50% [42/48] vs 64.81% [35/54], Log-Rankc 2=7.667, 7.063, P=0.006, 0.008). Low differentiation, TNM stage II-IV, and lymph node metastasis were independent risk factors for prognosis of patients with nasopharyngeal cancer. High expressions of miR-140-3p and AGT5 were protective factors for prognosis in patients with NPC (P<0.05). Conclusion: The downregulation of miR-140-3p and AGT5 expression in nasopharyngeal carcinoma patients is closely related to the occurrence, development, and prognosis of nasopharyngeal carcinoma via participating in the progression of nasopharyngeal carcinoma.

Key words： Nasopharyngeal cancer Micro RNA140-3p Autophagy related genes 5 Pathological features Prognostic

基金资助:陕西省级科技计划项目,(编号:2020JM1079)

通讯作者: 杨晓宇

引用本文:

黄昌群, 杨晓宇, 胡玮. 鼻咽癌组织中miR-140-3p AGT5表达变化及临床意义[J]. 河北医学, 2025, 31(2): 297-301.
HUANG Changqun, YANG Xiaoyu, HU Wei. Expression Changes and Clinical Significance of miR-140-3p AGT5 in Nasopharyngeal Carcinoma Tissue. HeBei Med, 2025, 31(2): 297-301.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.02.021 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I2/297

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