Abstract：Objective: To investigate the effects of curcumin on transforming growth factor-β (TGF-β)/Smad3 signaling pathway, wound healing, cellular inflammatory factors and angiogenesis in diabetic foot ulcer (DFU) rats. Methods: All rats were randomly divided into control group, DFU model group (DFU group), curcumin treatment group (curcumin group) and curcumin treatment +Smad3 inhibitor SIS3 group (SIS3 group). The wound healing rate of rats was calculated. The hematoxylin-eosin (H&E) staining was used to observe the pathological changes of wound tissue. The enzyme-linked immunosorbent assay (ELISA) was used to detect interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels. Western blot was used to analyze vascular endothelial growth factor (VEGF), CD31 and TGF-β1/Smad3 signaling protein expression. Results: Compared with those of control group, the rats in DFU group had significantly decreased wound healing rate and the protein expressions of TGF-β1 and Smad3, significantly increased the levels of IL-1β and TNF-α (P<0.05), and with a large number of inflammatory cells in the wound tissue and reduced neovascularization. Compared with those of DFU group, the rats in curcumin group presented significantly increased wound healing rate and the protein expressions of TGF-β1 and Smad3, significantly decreased the levels of IL-1β and TNF-α (P<0.05). The inflammatory cells in the wound tissue gradually decreased, and the number of new blood vessels gradually increased. After adding Smad3 inhibitor, the effect of curcumin on wound tissue injury in DFU rats can be eliminated to a certain extent. Conclusion: Curcumin may inhibit inflammatory response and induce angiogenesis by activating TGF-β1/Smad3 signaling pathway, thereby promoting wound healing in UFD rats.
闰景漠, 闰兴达, 高志胜, 李书林. 基于TGF-β1/Smad3信号通路观察姜黄素在糖尿病足溃疡伤口愈合中的作用[J]. 河北医学, 2024, 30(12): 1978-1982.
RUN Jingmo, et al. Effect of Curcumin on Wound Healing of Diabetic Foot Ulcer Based on TGF-β1/Smad3 Signaling Pathway. HeBei Med, 2024, 30(12): 1978-1982.
[1] Megallaa M H,Ismail A A,Zeitoun M H,et al.Association of diabetic foot ulcers with chronic vascular diabetic complications in patients with type 2 diabetes[J].Diabetes & Metabolic Syndrome:Clinical Research & Reviews,2019,13(2):1287-1292. [2] 梁斌,黄美健.姜黄素对慢性阻塞性肺疾病患者外周血内皮祖细胞血管生成能力的影响[J].中医杂志,2019,60(9):794-797. [3] Ghafouri-Fard S,Askari A,Shoorei H,et al.Antioxidant therapy against TGF-β/smads pathway involved in organ fibrosis[J].Cell Mol Med,2024,28(2):18052. [4] 李吉庆,林道斌,张永杰,等.裸花紫珠调控IGF-1/PI3K/Akt信号通路对糖尿病足溃疡大鼠创面愈合的影响[J].中国老年学杂志,2023,43(6):1458-1462. [5] 朱薇,张建华,孟繁亮,等.姜黄素对大鼠睾丸缺血再灌注损伤后的保护作用及核因子E2相关因子2表达的影响[J].解剖学杂志,2023,46(2):115-119. [6] Hizay A,Ozsoy U,Savas K,et al.Effect of ultrasound therapy on expression of vascular endothelial growth factor,vascular endothelial growth factor receptors,CD31 and functional recovery after facial nerve injury[J].Ultrasound in Medicine & Biology,2022,48(8):1453-1467. [7] Hu Y,Tao R,Chen L,et al.Exosomes derived from pioglitazone-pretreated MSCs accelerate diabetic wound healing through enhancing angiogenesis[J].Journal of Nanobiotechnology,2021,19(1):150. [8] Zhao Q,Xu J,Han X,et al.Growth differentiation factor 10 induces angiogenesis to promote wound healing in rats with diabetic foot ulcers by activating TGF-β1/Smad3 signaling pathway[J].Frontiers in Endocrinology,2023(13):1013018. [9] Yang X,Fan W,Huang R,et al.β-acetoxyisovaleryl alkannin (AAN-Ⅱ) from Alkanna tinctoria promotes the healing of pressure-induced venous ulcers in a rabbit model through the activation of TGF-β/Smad3 signaling[J].Cellular & Molecular Biology Letters,2021(26):1-11.
2024, Vol. 30 
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