Abstract：Objective: To analyze the mechanism of microRNA-138 in alleviating pelvic organ prolapse in rats by regulating the FBLN5/IL-1β pathway.Methods: Forty SPF grade SD female rats with three natural childbirths were selected, of which 10 were used as the control group, and another 30 were used to construct a pelvic organ prolapse model. After anesthesia, the vagina of the rats was opened and the urinary catheter was sutured to the vaginal opening to make it droop. Two weeks later, bilateral oophorectomy was performed. A total of 27 rats were successfully modeled and divided into model group (n=9), miRNA-138 inhibitor group (n=9), and miRNA-138 inhibitor+FBLN5 inhibitor group (n=9). The control group and model group were injected physiological saline, the miRNA-138 inhibitor group was injected 10μL of miR-138 inhibitor lentivirus suspension, and the miRNA-138 inhibitor+FBLN5 inhibitor group was injected 10 μ l of miR-138inhibitor lentivirus suspension. inhibitor+10μL FBLN5 inhibitor. The histopathological changes were observed, as well as fibroblast count, urodynamics, expression levels of type I and type Ⅲ collagen, FBLN5/IL-1β - related mRNA expression levels, and FBLN5/IL-1β expression levels in each group of rats.Results: Compared with the control group, the number of fibroblasts in the model decreased, and the basal bladder pressure, bladder voiding volume, bladder voiding pressure, bladder peak pressure, expression levels of type I collagen, type Ⅲ collagen, FBLN5 related mRNA expression, and FBLN5 expression decreased. The expression levels of IL-1β-related mRNA and IL-1β increased. Compared with the model group, the miRNA-138 inhibitor group and miRNA-138 inhibitor+FBLN5 inhibitor group had an increase in the number of fibroblasts and an increase in bladder basal pressure, bladder voiding volume, bladder voiding pressure, bladder peak pressure, type I collagen, type Ⅲ collagen expression, FBLN5 related mRNA expression, and FBLN5 expression. However, the expression of IL-1β-related mRNA and IL-1β-related mRNA decreased, and miRNA-138 inhibitor group had a larger number of fibroblasts. Bladder basal pressure, bladder voiding volume, bladder voiding pressure, bladder peak pressure, type I collagen, type Ⅲ collagen expression, FBLN5 related mRNA expression, and FBLN5 expression were higher, while IL-1β-related mRNA expression and IL-1β-related mRNA expression were low (P<0.05).Conclusion: Inhibiting the expression of microRNA-138 can regulate the FBLN5/IL-1β pathway, promote the expression of fibroblasts and collagen, improve rat urodynamics, and alleviate pelvic organ prolapse.
2024, Vol. 30 
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