黄芩苷调节TGF-β<sub>1</sub>/Smad信号通路对重症肺炎大鼠肺组织炎症反应的影响

doi:10.3969/j.issn.1006-6233.2024.12.05

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摘要 目的: 探讨黄芩苷(BC)对重症肺炎(SP)大鼠肺组织炎症反应及转化生长因子-β₁(TGF-β₁)/Smad信号通路的影响。方法: SD大鼠随机分为对照组(Control组)、模型组(SP组)、黄芩苷低、中、高剂量组(BC-L、BC-M、BC-H组)、黄芩苷高剂量+通路激活剂组(BC-H+SRI-011381组),每组18只,除对照组外,其余大鼠均构建SP大鼠模型;检测各组大鼠肺系数(LI)及湿重/干重比值(W/D);ELISA检测肺泡灌洗液中炎症因子水平及重症肺炎相关标志物降钙素原(PCT)、C反应蛋白(CRP)水平;瑞氏染色检测肺泡灌洗液沉淀物中中性粒细胞(NEU)、嗜酸性粒细胞(EOS)及淋巴细胞(LYM)数目;HE染色观察肺组织病理形态;Western blot检测TGF-β₁/Smad信号通路相关蛋白表达。结果: 与Control组比较,SP组肺组织结构遭到破坏,肺泡水肿部分可见出血,肺泡壁及肺间质增厚,大量炎症细胞浸润,LI、W/D、TNF-α、IL-1β、IL-6、PCT、CRP水平及NEU、EOS、LYM数目,TGF-β₁、p-Smad2/Smad2、p-Smad3/Smad3表达升高(P<0.05);与SP组比较,BC-L、BC-M、BC-H组肺组织结构受损现象得到改善,肺泡形态相对正常,水肿减轻,炎症细胞浸润减少,LI、W/D、TNF-α、IL-1β、IL-6、PCT、CRP水平及NEU、EOS、LYM数目,TGF-β₁、p-Smad2/Smad2、p-Smad3/Smad3表达降低(P<0.05);与BC-H组比较,BC-H+SRI-011381组肺组织结构破坏及肺泡水肿现象严重,炎症细胞浸润加剧,LI、W/D、TNF-α、IL-1β、IL-6、PCT、CRP水平及NEU、EOS、LYM数目,TGF-β₁、p-Smad2/Smad2、p-Smad3/Smad3表达升高(P<0.05)。结论: 黄芩苷可改善SP大鼠肺组织炎症,其作用机制与抑制TGF-β₁/Smad通路相关。

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	杨宗余
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关键词 ：黄芩苷, 转化生长因子-β₁/Smad信号通路, 重症肺炎

Abstract：Objective: To investigate the effect of baicalin (BC) on the inflammatory response of lung tissue and the transforming growth factor-β₁ (TGF-β₁)/Smad signaling pathway in severe pneumonia (SP) rats.Methods: SD rats were randomly separated into a control group, a model group (SP group), low, medium, and high-dose baicalin groups (BC-L, BC-M, BC-H groups), and a high-dose baicalin+pathway activator group (BC-H+SRI-011381 group), with 18 rats in each group. Except for the control group, all other rats were used to construct an SP rat model. The lung coefficient (LI) and wet/dry weight ratio (W/D) of rats in each group were measured. ELISA was applied to detect the levels of inflammatory factors and severe pneumonia related biomarkers procalcitonin (PCT) and C-reactive protein (CRP) in alveolar lavage fluid. Wright's staining was applied to detect the number of neutrophils (NEU), eosinophils (EOS), and lymphocytes (LYM) in the sediment of alveolar lavage fluid. HE staining was applied to observe the pathological morphology of lung tissue. Western blot was applied to detect the expression of TGF-β₁/Smad signaling pathway related proteins.Results: Compared with control group, SP group showed damage in lung tissue structure, with some areas of alveolar edema showing bleeding, thickening of alveolar walls and lung interstitium, and infiltration of a large number of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β₁, p-Smad2/Smad2, and p-Smad3/Smad3 were elevated (P<0.05). Compared with SP group, BC-L, BC-M, and BC-H groups showed improvement in lung tissue structure damage, with relatively normal alveolar morphology, reduced edema, and reduced infiltration of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β₁, p-Smad2/Smad2, and p-Smad3/Smad3 were reduced (P<0.05). Compared with BC-H group, BC-H+SRI-011381 group showed more severe damage to lung tissue structure and alveolar edema, with increased infiltration of inflammatory cells, the levels of LI, W/D, TNF-α, IL-1β, IL-6, PCT, CRP, the numbers of NEU, EOS, and LYM, and the expression of TGF-β₁, p-Smad2/Smad2, and p-Smad3/Smad3 were elevated (P<0.05).Conclusion: Baicalin can improve lung tissue inflammation in SP rats, and its mechanism of action is related to the inhibition of TGF-β₁/Smad pathway.

Key words： Baicalin TGF-β₁/Smad signaling pathway Severe pneumonia

基金资助:湖北省武汉市中医药科研项目,(编号:WZ22C58)

引用本文:

杨宗余, 蔡伟, 夏晨, 田辉. 黄芩苷调节TGF-β₁/Smad信号通路对重症肺炎大鼠肺组织炎症反应的影响[J]. 河北医学, 2024, 30(12): 1965-1971.
YANG Zongyu, CAI Wei, XIA Chen, et al. Effect of Baicalin on the Inflammatory Response of Lung Tissue in Severe Pneumonia Rats by Regulating the TGF-β₁/Smad Signaling Pathway. HeBei Med, 2024, 30(12): 1965-1971.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.12.05 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I12/1965

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