长链非编码 RNA UCA1调控食管鳞状细胞癌细胞生长和转移的功能研究

doi:10.3969/j.issn.1006-6233.2024.12.02

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摘要 目的: 探究长链非编码 RNA UCA1(LncRNA UCA1)对食管鳞状细胞癌细胞体内外生长和转移的影响。方法: 实时荧光定量PCR(Quantitative Real-time PCR,qRT-PCR)检测LncRNA UCA1在人食管上皮细胞HEEC和人食管鳞状细胞癌细胞EC109、EC9706、KYSE150和KYSE450中的表达,EC109和EC9706细胞分为si-NC组和si-UCA1组,CCK8法和流式细胞术分别检测细胞增殖和细胞周期,Transwell法检测细胞迁移和侵袭,Western blot检测细胞中E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、基质金属蛋白酶-9(Matrix Metalloprotein-9,MMP-9)相对表达量。1×10⁶个si-NC组和si-UCA1组EC109细胞接种于BALB/c-A 裸鼠左侧腋下,饲养4周后观察并记录小鼠肿瘤体积及瘤重。结果: EC109、EC9706、KYSE150和KYSE450中LncRNA UCA1表达显著高于HEEC细胞(P<0.001)。相比于si-NC组,si-UCA1组EC109和EC9706细胞增殖、迁移和侵袭能力显著降低(P<0.05),细胞周期显著阻滞(P<0.05),E-cadherin表达显著上调(P<0.001),Vimentin和MMP-9表达显著下调(P<0.001)。si-UCA1组小鼠肿瘤体积和瘤重显著低于si-NC组,(P<0.001)。结论: 抑制LncRNA UCA1可抑制食管鳞状细胞癌细胞增殖、迁移和侵袭能力以及在小鼠体内的生长。

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	唐成琼
	巴尔夏古丽·扎比胡拉
	陆艳荣

关键词 ：长链非编码RNA UCA1, 食管鳞状细胞癌, 转移

Abstract：Objective: To investigate the effects of LncRNA UCA1 on the in vivo and in vitro growth and metastasis of esophageal squamous cell carcinoma cells.Methods: qRT-PCR was used to detect the expression of LncRNA UCA1 in human esophageal epithelial cells (HEEC), human esophageal squamous cell carcinoma cells (EC109, EC9706, KYSE150, and KYSE450). EC109 and EC9706 cells were divided into si-NC group and si-UCA1 group. CCK8 and flow cytometry were used to detect cell proliferation and cell cycle, Transwell method was used to detect cell migration and invasion. Western blot was used to detect the relative expression levels of E-cadherin, Vimentin, and MMP-9 in cells. 1×10⁶ EC109 cells of si-NC and si-UCA1 groups were inoculated into the left axilla of BALB/c-A nude mice, after 4 weeks of feeding, the tumor volume and weight of the mice were observed and recorded.Results: The expression of LncRNA UCA1 in EC109, EC9706, KYSE150, and KYSE450 was significantly higher than that in HEEC cells (P<0.001). Compared with the si-NC group, the proliferation, migration, and invasion abilities of EC109 and EC9706 cells in the si-UCA1 group were significantly reduced (P<0.05), cell cycle was significantly arrested (P<0.05), the expression of E-cadherin was significantly upregulated (P<0.001), and the expression of Vimentin and MMP-9 were significantly downregulated (P<0.001). The tumor volume and weight of mice in the si-UCA1 group were significantly lower than those in the si-NC group (P<0.001). Conclusion: Inhibiting LncRNA UCA1 can inhibit the proliferation, migration, and invasion ability of esophageal squamous cell carcinoma cells, as well as their growth in mice.

Key words： LncRNA UCA1 Esophageal squamous cell carcinoma Metastasis

基金资助:新疆维吾尔自治区自然基金面上项目,(编号:2022D01C300)

通讯作者: 陆艳荣

引用本文:

唐成琼, 巴尔夏古丽·扎比胡拉, 陆艳荣. 长链非编码 RNA UCA1调控食管鳞状细胞癌细胞生长和转移的功能研究[J]. 河北医学, 2024, 30(12): 1945-1951.
TANG Chengqiong, Baerxiaguli Zabihula, LU Yanrong. Study on the Regulation of Growth and Metastasis of Esophageal Squamous Cell Carcinoma Cells by LncRNA UCA1. HeBei Med, 2024, 30(12): 1945-1951.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.12.02 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I12/1945

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