黄芩苷调节SDF-1/CXCR4信号通路对高糖诱导的心肌细胞凋亡的影响

doi:10.3969/j.issn.1006-6233.2024.11.06

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摘要 目的:探讨黄芩苷调节基质细胞衍生因子1(SDF-1)/趋化因子受体4(CXCR4)信号通路对高糖(HG)诱导的心肌细胞凋亡的影响。方法:以H9C2心肌细胞为研究对象,分为对照组、HG组、黄芩苷低浓度组(L-Ba组)、黄芩苷中浓度组(M-Ba组)、黄芩苷高浓度组(H-Ba组)、黄芩苷+CXCR4抑制剂组(Ba+AMD3100组)。CCK-8法检测各组细胞活力;流式细胞仪检测各组细胞凋亡率;qRT-PCR检测各组细胞中Bax、Bcl-2的mRNA表达;微板法检测各组细胞中SOD活力和MDA含量;免疫印迹法(WB)检测各组细胞中SDF-1、CXCR4蛋白表达水平。结果:与对照组比较,HG组存活率、Bcl-2 mRNA相对表达量、SOD活性及SDF-1和CXCR4蛋白表达水平降低,心肌细胞凋亡率、Bax mRNA相对表达量及MDA含量升高,差异均有统计学意义(P<0.05);与HG组比较,L-Ba组、M-Ba组、H-Ba组存活率、Bcl-2 mRNA相对表达量、SOD活性及SDF-1和CXCR4蛋白表达水平升高,心肌细胞凋亡率、Bax mRNA相对表达量及MDA含量降低,且随着黄芩苷浓度增加呈逐渐上升或下降趋势,差异均有统计学意义(P<0.05);Ba+AMD3100组心肌细胞存活率、Bcl-2 mRNA相对表达量、SOD活性、SDF-1、CXCR4蛋白表达水平低于L-Ba组、M-Ba组、H-Ba组,心肌细胞凋亡率、Bax mRNA相对表达量、MDA含量高于L-Ba组、M-Ba组、H-Ba组,差异均有统计学意义(P<0.05)。结论:黄芩苷可通过激活SDF-1/CXCR4信号通路缓解HG诱导的心肌细胞凋亡。

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	郭静
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关键词 ：黄芩苷, 基质细胞衍生因子1, 趋化因子受体4, 高糖, 心肌细胞, 凋亡

Abstract：Objective: To investigate the effect of baicalin on high glucose (HG) induced apoptosis of myocardial cells by regulating the stromal cell derived factor 1 (SDF-1)/chemokine receptor 4 (CXCR4) signaling pathway.Methods: H9C2 myocardial cells were used as the research object and grouped into control group, HG group, low concentration baicalin group (L-Ba group), medium concentration baicalin group (M-Ba group), high concentration baicalin group (H-Ba group), and baicalin+CXCR4 inhibitor group (Ba+AMD3100 group). CCK-8 method was applied to detect cell viability in each group; flow cytometry was applied to detect the apoptosis rate of cells in each group; qRT-PCR was applied to detect the mRNA expression of Bax and Bcl-2 of cells in each group; microplate method was applied to detect SOD activity and content of MDA of cells in each group; Western blot (WB) was applied to detect the expression levels of SDF-1 and CXCR4 proteins of cells in each group.Results: Compared with the control group, the survival rate, relative expression of Bcl-2 mRNA, SOD activity, and expression levels of SDF-1 and CXCR4 proteins in the HG group decreased, the apoptosis rate of myocardial cells, relative expression of Bax mRNA, and content of MDA increased (P<0.05); compared with the HG group, the survival rate, relative expression of Bcl-2 mRNA, SOD activity, and expression levels of SDF-1 and CXCR4 proteins in the L-Ba group, M-Ba group, H-Ba group increased, the apoptosis rate of myocardial cells, relative expression of Bax mRNA, and content of MDA decreased, and showed gradual upward or downward trend as the concentration of baicalin increased (P<0.05); the survival rate of myocardial cells, relative expression of Bcl-2 mRNA, SOD activity, and protein expression levels of SDF-1 and CXCR4 in the Ba+AMD3100 group were lower than those in the L-Ba, M-Ba, and H-Ba groups, the apoptosis rate of myocardial cells, relative expression of Bax mRNA, and content of MDA were higher in the L-Ba group, M-Ba group, and H-Ba group (P<0.05).Conclusion: Baicalin can alleviate HG-induced apoptosis of myocardial cells by activating the SDF-1/CXCR4 signaling pathway.

Key words： Baicalin Stromal cell derived factor 1 Chemokine receptor 4 High glucose Myocardial cells Apoptosis

基金资助:湖北省荆门市引导性科研计划项目,(编号:2023YDKY086)

通讯作者: 杨举红

引用本文:

郭静, 杨举红, 张琳, 郑梅. 黄芩苷调节SDF-1/CXCR4信号通路对高糖诱导的心肌细胞凋亡的影响[J]. 河北医学, 2024, 30(11): 1792-1796.
GUO Jing, YANG Juhong, ZHANG Lin, et al. Effect of Baicalin on High-Glucose Induced Apoptosis of Myocardial Cells by Regulating the SDF-1/CXCR4 Signaling Pathway. HeBei Med, 2024, 30(11): 1792-1796.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.11.06 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I11/1792

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