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河北医学  2024, Vol. 30 Issue (11): 1772-1777    DOI: 10.3969/j.issn.1006-6233.2024.11.03
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金合欢素调节NF-κB信号通路对幽门螺旋菌感染小鼠胃黏膜损伤的研究
梁磊, 贾纯亮, 李青科, 马国明, 王剑, 李翰嵩, 张磊
河北省唐山市人民医院胃肠外一科, 河北 唐山 063000
The Effect of Acacetin on Gastric Mucosal Injury in Helicobacter Pylori-Infected Mice by Regulating NF-κB Signaling Pathway
LIANG Lei, JIA Chunliang, LI Qingke, et al
Tangshan People's Hospital, Hebei Tangshan 063000, China
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摘要 目的:探讨金合欢素调节NF-κB信号通路对幽门螺旋菌(Hp)感染小鼠胃黏膜损伤的影响及机制。方法:C57BL/6小鼠以109 CFU/mL Hp(0.3mL)灌胃,并将建模成功小鼠随机分为Hp组、金合欢素低(金合欢素-L,30mg/kg)、高剂量(金合欢素-H,75mg/kg)组、阳性对照[奥美拉唑(400μg/kg)、克拉霉素(7.15mg/kg)及阿莫西林(14.25mg/kg)]组以及金合欢素-H+Prostratin(75mg/kg金合欢素+0.5mg/kg Prostratin)组,并以灌胃0.3mL生理盐水的小鼠为对照组,随后检测血清中胃肠激素[胃动素(MTL),胃饥饿素(Ghrelin),5-色羟胺(5-HT)]指标;分离胃组织,检测其病理学变化、评分,并检测组织中白细胞介素(IL)-1β、IL-10、肿瘤坏死因子(TNF)-α水平、闭锁蛋白(Occludin),闭锁小带蛋白(ZO-1) mRNA表达、细胞凋亡变化及凋亡蛋白Bax、Bcl-2、p-NF-kB p65、NF-kB p65表达。结果:与对照组相比,Hp组Ghrelin水平、IL-10水平、Occludin、ZO-1 mRNA表达、Bcl-2表达显著降低,MTL、5-HT水平、损伤评分、IL-1β、TNF-α水平、细胞凋亡率、Bax、p-NF-kB p65/NF-kB p65表达显著增加(P<0.05);与Hp组相比,金合欢素-L、金合欢素-H组、阳性对照组Ghrelin水平、IL-10水平、Occludin、ZO-1 mRNA表达、Bcl-2表达显著增加,MTL、5-HT水平、损伤评分、IL-1β、TNF-α水平、细胞凋亡率、Bax、p-NF-kB p65/NF-kB p65表达显著降低,不同金合欢素组间有差异(P<0.05);与金合欢素-H组相比,金合欢素-H+Prostratin组Ghrelin水平、IL-10水平、Occludin、ZO-1 mRNA表达、Bcl-2表达显著降低,MTL、5-HT水平、损伤评分、IL-1β、TNF-α水平、细胞凋亡率、Bax、p-NF-kB p65/NF-kB p65表达显著增加(P<0.05)。结论:金合欢素可通过NF-κB信号通路改善Hp感染小鼠胃黏膜损伤。
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关键词 金合欢素NF-κB信号通路幽门螺旋菌胃黏膜    
AbstractObjective: To investigate the effect of Acacetin on gastric mucosal injury in Helicobacter pylori (Hp)-infected mice by regulating NF-κB signaling pathway.Methods: C57BL/6 mice were given 109 CFU/mL Hp (0.3 mL) gavage, and the successfully modeled mice were randomly divided into Hp group, low Acacetin (Acacetin-L, 30 mg/kg), high dose (Acacetin-H, 75 mg/kg) group, positive control [omeprazole (400 μg/kg), clarithromycin (7.15 mg/kg) and amoxicillin (14.25 mg/kg)] group, and Acacetin-H+Prostratin (75 mg/kg Acacetin+ 0.5 mg/kg Prostratin) group, and mice given 0.3 mL of normal saline by gavage were made the control group. Gastrointestinal hormones [motilin (MTL), Ghrelin, 5-tryptamine (5-HT)] in serum were measured. Gastric tissue was isolated and the pathological changes and scores were detected. The levels of interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α, mRNA expression of occludin (Occludin), atresia band protein (ZO-1), apoptosis and the expression of apoptotic proteins Bax, Bcl-2, p-NF-kB p65 and NF-kB p65 were detected.Results: Compared with the control group, the Ghrelin level, IL-10 level, Occludin, ZO-1 mRNA expression and Bcl-2 expression in the Hp group were greatly reduced, the MTL, 5-HT levels, damage score, IL-1β, TNF-α levels, apoptosis rate, Bax, p-NF-kB p65/NF-kB p65 expression were greatly increased (P<0.05). Compared with the Hp group, the Ghrelin level, IL-10 level, Occludin, ZO-1 mRNA expression and Bcl-2 expression in the Acacetin-L, Acacetin-H group, positive control group were greatly increased, the MTL, 5-HT levels, damage score, IL-1β, TNF-α levels, apoptosis rate, Bax, p-NF-kB p65/NF-kB p65 expression were greatly reduced, there were differences among different Acacetin groups (P<0.05). Compared with the Acacetin-H group, the Ghrelin level, IL-10 level, Occludin, ZO-1 mRNA expression and Bcl-2 expression in the Acacetin-H+Prostratin group were greatly decreased, the MTL, 5-HT levels, damage score, IL-1β, TNF-α levels, apoptosis rate, Bax, p-NF-kB p65/NF-kB p65 expression were greatly increased (P<0.05).Conclusion: Acacetin can improve gastric mucosal injury in Hp infected mice through NF-κB signaling pathway.
Key wordsAcacetin    NF-κB signaling pathway    Helicobacter pylori    Gastric mucosa
    
基金资助:河北省医学科学研究课题计划项目,(编号:20201528)
引用本文:   
梁磊, 贾纯亮, 李青科, 马国明, 王剑, 李翰嵩, 张磊. 金合欢素调节NF-κB信号通路对幽门螺旋菌感染小鼠胃黏膜损伤的研究[J]. 河北医学, 2024, 30(11): 1772-1777.
LIANG Lei, JIA Chunliang, LI Qingke, et al. The Effect of Acacetin on Gastric Mucosal Injury in Helicobacter Pylori-Infected Mice by Regulating NF-κB Signaling Pathway. HeBei Med, 2024, 30(11): 1772-1777.
链接本文:  
http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.11.03     或     http://www.hbyxzzs.cn/CN/Y2024/V30/I11/1772
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