Abstract：Objective: To investigate the effect of Acacetin on gastric mucosal injury in Helicobacter pylori (Hp)-infected mice by regulating NF-κB signaling pathway.Methods: C57BL/6 mice were given 109 CFU/mL Hp (0.3 mL) gavage, and the successfully modeled mice were randomly divided into Hp group, low Acacetin (Acacetin-L, 30 mg/kg), high dose (Acacetin-H, 75 mg/kg) group, positive control [omeprazole (400 μg/kg), clarithromycin (7.15 mg/kg) and amoxicillin (14.25 mg/kg)] group, and Acacetin-H+Prostratin (75 mg/kg Acacetin+ 0.5 mg/kg Prostratin) group, and mice given 0.3 mL of normal saline by gavage were made the control group. Gastrointestinal hormones [motilin (MTL), Ghrelin, 5-tryptamine (5-HT)] in serum were measured. Gastric tissue was isolated and the pathological changes and scores were detected. The levels of interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α, mRNA expression of occludin (Occludin), atresia band protein (ZO-1), apoptosis and the expression of apoptotic proteins Bax, Bcl-2, p-NF-kB p65 and NF-kB p65 were detected.Results: Compared with the control group, the Ghrelin level, IL-10 level, Occludin, ZO-1 mRNA expression and Bcl-2 expression in the Hp group were greatly reduced, the MTL, 5-HT levels, damage score, IL-1β, TNF-α levels, apoptosis rate, Bax, p-NF-kB p65/NF-kB p65 expression were greatly increased (P<0.05). Compared with the Hp group, the Ghrelin level, IL-10 level, Occludin, ZO-1 mRNA expression and Bcl-2 expression in the Acacetin-L, Acacetin-H group, positive control group were greatly increased, the MTL, 5-HT levels, damage score, IL-1β, TNF-α levels, apoptosis rate, Bax, p-NF-kB p65/NF-kB p65 expression were greatly reduced, there were differences among different Acacetin groups (P<0.05). Compared with the Acacetin-H group, the Ghrelin level, IL-10 level, Occludin, ZO-1 mRNA expression and Bcl-2 expression in the Acacetin-H+Prostratin group were greatly decreased, the MTL, 5-HT levels, damage score, IL-1β, TNF-α levels, apoptosis rate, Bax, p-NF-kB p65/NF-kB p65 expression were greatly increased (P<0.05).Conclusion: Acacetin can improve gastric mucosal injury in Hp infected mice through NF-κB signaling pathway.
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2024, Vol. 30 
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