Abstract：Objective: To investigate the effects of exosomal circ_001895 transportation on endothelial progenitor cells under high glucose stress derived from patients with type 2 diabetes (T2DM). Methods: Serum samples were obtained from T2DM patients and divided into serum group and EXO group. The expression levels of circ_001895 in serum and EXO were detected by using RT-qPCR technology. EXOs derived from T2DM patients' serum with significantly elevated circ_001895 expression were named EXO-circ. Endothelial progenitor cells provided by Wuhan Procell were cultured and divided into the following three groups. Control Group: Cultured conventionally for 24 hours without additional treatment. High Glucose (HG) Group: Treated with 30 mmoL/L glucose for 24 hours. HG+EXO-circ Group: Treated with 30 mmoL/L glucose combined with 100μg/mL EXO-circ for 24 hours. The expression of circ_001895 in each group of cells was detected using RT-qPCR technology. Cell proliferation vitality was detected using the CCK-8 method. Cell apoptosis was detected using flow cytometry. The expression of angiogenic markers E-cadherin, N-cadherin, and Vimentin was detected using Western blot technology. Cell migration capacity was detected using the Transwell cell migration assay. Results: Compared to the serum group, the relative expression level of circ_001895 in the EXO group was significantly upregulated (P<0.05).Compared to the control group, the relative expression levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG group were significantly upregulated (all P<0.05), while cell proliferation vitality, migration capacity, and the relative expression levels of N-cadherin and Vimentin were significantly downregulated (all P<0.05). Compared to the HG group, the relative expression levels of circ_001895 and E-cadherin and the cell apoptosis rate in the HG+EXO-circ group were significantly upregulated (all P<0.05), while cell proliferation vitality, migration capacity, and the relative expression levels of N-cadherin and Vimentin were significantly downregulated (all P<0.05). Conclusion: Transportation of circ_001895 in exosomes from T2DM inhibits proliferation, migration, and vascular generation of endothelial progenitor cells under high glucose stress, while promoting apoptosis.
张洁, 张丽, 王晨菲, 罗荔. 2型糖尿病来源外泌体转运circ_001895对高糖胁迫下内皮祖细胞功能的影响[J]. 河北医学, 2024, 30(10): 1592-1596.
ZHANG Jie, ZHANG Li, WANG Chenfei, et al. Effect of Exosomal Transport of circ_001895 from Type 2 Diabetes Mellitus on Endothelial Progenitor Cells under High Glucose Stress. HeBei Med, 2024, 30(10): 1592-1596.
[1] Afsharmanesh M R,Mohammadi Z,Mansourian A R,et al.A Review of micro RNAs changes in T2DM in animals and humans [J].Diabetes,2023,15(8): 649-664. [2] Thummasorn S,Apichai S,Chupradit S,et al.T2DM patients with depression have higher levels of hyperglycemia and cognitive decline than T2DM patients [J].Plos One,2022,17(8):273327. [3] Rai V,Moellmer R,Agrawal D K.Clinically relevant experimental rodent models of diabetic foot ulcer [J].Mol Cell Biochem,2022,477(4): 1239-47. [4] Du X,Lv J,Feng J,et al.Plasma exosomes lncRNA-miRNA-mRNA network construction and its diagnostic efficacy identification in first-episode schizophrenia [J].BMC Psychiatry,2023,23(1): 611. [5] Ma J,Wang P,Huang L,et al.Bioinformatic analysis reveals an exosomal miRNA-mRNA network in colorectal cancer [J].BMC Med Genomics,2021,14(1): 60. [6] Chen Z,Xiao K,Chen S,et al.Circular RNA hsa_circ_001895 serves as a sponge of microRNA-296-5p to promote clear cell renal cell carcinoma progression by regulating SOX12 [J].Cancer Sci,2020,111(2): 713-726. [7] 赵能江,张智海,陈薇,等.《中国2型糖尿病防治指南(2020年版)》亮点解读及糖尿病中医指南分析[J].中国中西医结合杂志,2021,41(6): 652-655. [8] Liang Z H,Lin S S,Pan N F,et al.UCMSCs-derived exosomal circHIPK3 promotes ulcer wound angiogenesis of diabetes mellitus via miR-20b-5p/Nrf2/VEGFA axis [J].Diabet Med,2023,40(2): 14968. [9] Xiong Y,Chen L,Liu P,et al.All-in-One: multifunctional hydrogel accelerates oxidative diabetic wound healing through timed-release of exosome and fibroblast growth factor [J].Small,2022,18(1): 2104229. [10] An Y,Lin S,Tan X,et al.Exosomes from adipose-derived stem cells and application to skin wound healing [J].Cell Prolif,2021,54(3): 12993. [11] Wei X B,Jiang W Q,Zeng J H,et al.Exosome-derived LncRNA NEAT1 exacerbates sepsis-associated encephalopathy by promoting ferroptosis through regulating miR-9-5p/TFRC and GOT1 axis [J].Mol Neurobiol,2022,59(3): 1954-1969. [12] Krylova S V,Feng D.The machinery of exosomes: biogenesis,release and uptake [J].Int Mol Sci,2023,24(2):1337. [13] Johnson J,Law S Q K,Shojaee M,et al.First-in-human clinical trial of allogeneic,platelet-derived extracellular vesicles as a potential therapeutic for delayed wound healing [J].Extracell Vesicles,2023,12(7): 12332. [14] Han X,Wu P,Li L,et al.Exosomes derived from autologous dermal fibroblasts promote diabetic cutaneous wound healing through the Akt/β-catenin pathway [J].Cell Cycle,2021,20(5-6): 616-629.
2024, Vol. 30 
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