Abstract：Objective: To investigate the effects of long non-coding RNA 00852 (LINC00852) on the proliferation, invasion, and migration of non-small cell lung cancer (NSCLC) cells by regulating the microRNA-671-5p (miR-671-5p)/myosin heavy chain 9 (MYH9) signaling axis. Methods: The expression levels of LINC00852, miR-671-5p, and MYH9 mRNA were detected in NSCLC tissues and adjacent normal tissues using qRT-PCR. A549 cells were randomly divided into several groups: Control group, sh-NC group, sh-LINC00852 group, sh-LINC00852+anti-NC group, and sh-LINC00852+anti-miR-671-5p group. The expression levels of LINC00852, miR-671-5p, and MYH9 mRNA were detected. Cell proliferation, invasion, and migration were assessed by colony formation assay, Transwell assay, and wound healing assay, respectively. Western blot was used to detect the protein expression of PCNA, MYH9, and MMP-9. Dual-luciferase reporter assay was performed to verify the interaction between LINC00852 and miR-671-5p, and between miR-671-5p and MYH9. Results: The expression levels of LINC00852 and MYH9 mRNA were significantly higher in NSCLC tissues compared with adjacent normal tissues, while the expression of miR-671-5p was significantly lower. Knockdown of LINC00852 inhibited the proliferation, invasion, and migration of A549 cells, and downregulated the expression of MYH9, PCNA, and MMP-9, while upregulating the expression of miR-671-5p. Dual-luciferase reporter assay confirmed that LINC00852 could directly target miR-671-5p, and miR-671-5p could directly target MYH9. Conclusion: LINC00852 inhibits the proliferation, invasion, and migration of NSCLC cells by regulating the miR-671-5p/MYH9 signaling axis.
李亚明, 张进召, 刘松, 潘双, 刘佳银. LINC00852调节miR-671-5p/MYH9信号轴对非小细胞肺癌细胞增殖侵袭及迁移的影响[J]. 河北医学, 2024, 30(10): 1585-1591.
LI Yaming, ZHANG Jinzhao, LIU Song, et al. Effect of LINC00852 Regulating the miR-671-5p/MYH9 Signaling Axis on Proliferation Invasion and Migration of Non-Small Cell Lung Cancer Cells. HeBei Med, 2024, 30(10): 1585-1591.
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2024, Vol. 30 
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