Abstract：Objective: To explore the effect of plantamajoside (PMS) on the inflammatory response of human gingival fibroblasts induced by lipopolysaccharide (LPS) by inhibiting nuclear factor-κB (NF-κB)/interleukin-6 (IL-6) signaling pathway.Methods: The cultured human gingival fibroblasts (HGnF) were randomly divided into control group, model group (10mg/L LPS), PMS low and high-dose groups (50, 100μg/mL PMS+10mg/L LPS), activator group (10μM BAY11-7085+10mg/L LPS), and PMS high-dose + activator group (10μM BAY11-7085+100μg/mL+10mg/L LPS). CCK-8 method was used to detect HGnF cell viability, ELISA method was used to detect the contents of interleukin 1β (IL-1β), interleukin 6 (IL-6), and interleukin 18 (IL-18) in the supernatant of HGnF cells. Western blot method was used to detect the expression levels of inducible nitric oxide synthase (iNOS), IL-1β and NF-κB/IL-6 signaling pathway-related proteins.Results: Low and high-doses PMS could increase the cell viability of HGnF induced by LPS, decrease the contents of IL-1β, IL-6, IL-18 in supernatant, and the expression levels of IL-1β, iNOS, p-NF-κB/NF-κB, and IL-6 in cells (P<0.05). The activator BAY11-7085 reversed the effects of high-dose PMS on the above indicators in LPS-induced HGnF cells (P<0.05). Conclusion: PMS may improve the inflammation of HGnF cells induced by LPS by inhibiting the NF-κB/IL-6 signaling pathway.
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2024, Vol. 30 
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