芍药苷调节SIRT1/PGC-1α/Nrf2信号通路对过氧化氢诱导皮肤成纤维细胞氧化应激损伤的影响

doi:10.3969/j.issn.1006-6233.2024.09.03

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摘要 目的: 探讨芍药苷(PF)调节沉默信息调节因子2同系物1(SIRT1)/氧化物酶体增殖物激活受体γ共激活剂-1α(PGC-1α)/核因子E2相关因子2(Nrf2)信号通路对过氧化氢(H₂O₂)诱导皮肤成纤维细胞(HSF)氧化应激损伤的影响。方法: 以HSF细胞为研究对象,将其分为Control组、H₂O₂组、L-PF、M-PF、H-PF组、PF+EX527组;CCK-8检测HSF细胞增殖;β-半乳糖苷酶染色法检测HSF细胞衰老;流式细胞仪检测HSF细胞凋亡;ELISA法检测HSF细胞中ROS、SOD、GSH、MDA的表达;WB检测HSF细胞中SIRT1、PGC-1α、Nrf2蛋白表达。结果: H₂O₂组HSF细胞的存活率、SOD、GSH、SIRT1、PGC-1α、Nrf2表达低于Control组,衰老细胞比例、凋亡率、ROS、MDA表达高于Control组(P<0.05);与H₂O₂组比较,L-PF组、M-PF组、H-PF组存活率、SOD、GSH、SIRT1、PGC-1α、Nrf2表达升高,衰老细胞比例、凋亡率、ROS、MDA表达降低(P<0.05);PF+EX527组存活率、SOD、GSH、SIRT1、PGC-1α、Nrf2表达低于H-PF组,衰老细胞比例、凋亡率、ROS、MDA表达高于H-PF组(P<0.05)。结论: PF可以抑制H₂O₂诱导的HSF细胞氧化应激损伤,其机制可能是激活SIRT1/PGC-1α/Nrf2信号通路实现的。

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	黄燕
	杨艳清
	周进飞

关键词 ：芍药苷, 沉默信息调节因子2同系物1, 氧化物酶体增殖物激活受体γ共激活剂-1α, 核因子E2相关因子2, 皮肤成纤维细胞, 氧化应激损伤

Abstract：Objective: To investigate the effect of paeoniflorin (PF) on oxidative stress damage induced by hydrogen peroxide (H₂O₂) in skin fibroblasts (HSF) by regulating the silent information regulator 2 homologue 1 (SIRT1)/peroxisome proliferator activated receptor-gamma coactivator-1α (PGC-1α)/nuclear factor erythroid-derived 2-related factor 2 (Nrf2) signaling pathway.Methods: HSF cells were studied and separated into the Control group, H₂O₂ group, L-PF, M-PF, H-PF group, and PF+EX527 group. CCK-8 was applied to detect HSF cell proliferation. The β-galactosidase staining method was applied to detect HSF cell aging. Flow cytometry was used to detect apoptosis of HSF cells. ELISA method was used to detect the expression of ROS, SOD, GSH, and MDA in HSF cells. WB was used to detect the expressions of SIRT1, PGC-1α, and Nrf2 proteins in HSF cells.Results: The survival rate, SOD, GSH, SIRT1, PGC-1α, and Nrf2 expression of HSF cells in the H₂O₂ group were lower than in the control group, the proportion of aging cells, apoptosis rate, ROS, and MDA expression were higher than in the control group (P<0.05). Compared with the H₂O₂ group, the survival rate, SOD, GSH, SIRT1, PGC-1α, and Nrf2 expression were increased in the L-PF group, M-PF group, and H-PF group, the proportion of aging cells, apoptosis rate, ROS, and MDA expression were decreased (P<0.05). The survival rate, SOD, GSH, SIRT1, PGC-1α, and Nrf2 expression in the PF+EX527 group were lower than in the H-PF group, the proportion of aging cells, apoptosis rate, ROS, and MDA expression were higher than in the H-PF group (P<0.05).Conclusion: PF can inhibit H₂O₂-induced oxidative stress damage in HSF cells, and its mechanism may be achieved by activating the SIRT1/PGC-1α/Nrf2 signaling pathway.

Key words： Paeoniflorin Silent information regulator 2 homologue 1 Peroxisome proliferator activated receptor-gamma coactivator-1α Nuclear factor erythroid-derived 2-related factor 2 Skin fibroblasts Oxidative stress damage

基金资助:湖北省武汉市医学科研项目立项任务书,(编号:WX20Q03)

引用本文:

黄燕, 杨艳清, 周进飞. 芍药苷调节SIRT1/PGC-1α/Nrf2信号通路对过氧化氢诱导皮肤成纤维细胞氧化应激损伤的影响[J]. 河北医学, 2024, 30(9): 1428-1433.
HUANG Yan, YANG Yanqing, et al. Effect of Paeoniflorin on Hydrogen Peroxide-Induced Oxidative Stress Damage in Skin Fibroblasts by Regulating the SIRT1/PGC-1α/Nrf2 Signaling Pathway. HeBei Med, 2024, 30(9): 1428-1433.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.09.03 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I9/1428

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