Expression Levels of miR-155 and miR-132 in Peripheral Blood of Patients with Diabetic Foot Infection and Their Correlation with the Degree of Lower Limb Vascular Lesions
ZHANG Lin, DONG Peng, WANG Ning, et al
The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi Xi'an 710004, China
Abstract：Objective: To explore the expression levels of peripheral blood miR-155 and miR-132 in patients with diabetic foot (DF) infection and the relationship with lower extremity vascular lesion degree. Methods: A total of 62 patients with simple type 2 diabetes mellitus (T2DM) in the hospital from June 2020 to June 2023 were selected and included in T2DM group, and 93 patients with T2DM complicated with DF infection during the same period were enrolled as DF infection group. The levels of peripheral blood miR-155 and miR-132 were compared, and the relationship between levels of peripheral blood miR-155 and miR-132 and clinical characteristics in patients with DF infection was analyzed. According to the classification criteria of the Transatlantic Association of Society Consensus (TASC), patients with DF infection were classified into grades A, B, C, and D. The levels of miR-155 and miR-132 in peripheral blood were compared among patients with DF infection and different lower extremity vascular lesion degrees. The Spearman method was used to analyze the correlation between peripheral blood miR-155 and miR-132 and lower extremity vascular lesion degree. Results: The level of miR-155 in DF infection group was higher than that in T2DM group while the level of miR-132 was lower than that in T2DM group (all P<0.05). The course of DF ulcer, wound area, and proportion of Wagner grade IV in the high miR-155 expression group were higher than those in the low expression group, while the course of DF ulcer, wound area, and proportion of Wagner grade IV were lower in high miR-132 expression group than those in low expression group, and the healing rate of DF ulcer after 8 weeks was higher than that in low expression group (all P<0.05). The miR-155 level in patients with DF infection of grade A to D was increased in turn while the miR-132 level was decreased in turn (all P<0.05). Spearman analysis showed that the expression level of miR-155 was positively correlated with lower extremity vascular lesion degree, while the expression level of miR-132 was negatively correlated with lower extremity vascular degree (all P<0.05). Conclusion: The high expression of miR-155 in the peripheral blood of patients with DF infection is positively correlated with degree of lower extremity vascular lesion, and the low expression of miR-132 is negatively correlated with the degree of lower extremity vascular lesion.
张琳, 董鹏, 汪宁, 张若, 孔颖, 薛阳利, 魏玲云, 李友芳. 糖尿病足感染患者外周血miR-155及miR-132表达水平及其与下肢血管病变程度的关系分析[J]. 河北医学, 2024, 30(7): 1156-1160.
ZHANG Lin, DONG Peng, WANG Ning, et al. Expression Levels of miR-155 and miR-132 in Peripheral Blood of Patients with Diabetic Foot Infection and Their Correlation with the Degree of Lower Limb Vascular Lesions. HeBei Med, 2024, 30(7): 1156-1160.
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2024, Vol. 30 
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