Abstract：Objective: To investigate the impact of honeysuckle polyphenol crude extract on the malignant biological behavior of non-small cell lung cancer (NSCLC) NCI-H1299 cells and the molecular mechanisms involved. Methods: NCI-H1299 cells were divided into NC group, paclitaxel group, and low, medium, and high-dose groups of honeysuckle polyphenol crude extract. Additionally, miR-100-5p group, miR-NC group, anti-miR-100-5p + high-dose group, and anti-miR-NC + high-dose group were established. MTT assay was used to detect cell viability, Transwell assay for cell migration and invasion, Western blot for E-Cadherin, N-Cadherin, Vimentin, and Wnt/β-catenin pathway-related protein expression, and real-time fluorescence quantitative PCR (RT-qPCR) for miR-100-5p expression. Results: After treatment with different concentrations of honeysuckle polyphenol crude extract, NCI-H1299 cell viability decreased, and the number of cell migration and invasion decreased. E-Cadherin and miR-100-5p expression levels increased, while N-Cadherin and Vimentin expression levels decreased (P<0.05). Overexpression of miR-100-5p increased NCI-H1299 cell viability, cell migration [(226±20.03) cells], and invasion [(171±15.03) cells], while E-Cadherin expression level decreased (0.34±0.03), and N-Cadherin (0.88±0.08) and Vimentin expression levels increased (P<0.05). Downregulating miR-100-5p could reverse the effects of honeysuckle polyphenol crude extract on NCI-H1299 cell proliferation, migration, invasion, and the Wnt/β-catenin pathway. Conclusion: Honeysuckle polyphenol crude extract may inhibit the proliferation, migration, and invasion of NSCLC NCI-H1299 cells by upregulating miR-100-5p expression, thereby suppressing the activation of the Wnt/β-catenin signaling pathway.
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2024, Vol. 30 
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