Abstract：Objective: To screen serum exosomal long non-coding RNA (LncRNA) as potential molecular markers for the early diagnosis of ankylosing spondylitis (AS). Methods: Whole blood samples of AS patients in our hospital from January 2021 to December 2022 were collected. Exosomes were isolated from the plasma of AS patients (n=50) and healthy individuals (n=50) by ultrafast centrifuge. Total exosomal RNA was extracted using TRIzol reagent. LncRNAs were analyzed by microarray analysis in the two groups of patients, and the expression levels of LncRNAs in all patients and healthy subjects were measured by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The results of this study showed that six lncrnas (LNCV6_116109、LNCV6_98390、LNCV6_38772、LNCV6_108266、LNCV6_84003和LNCV6_98602) were significantly up-regulated in AS patients by qRT-PCR. Not a healthy individual. Receiver operating characteristic curve (ROC) was used to verify its diagnostic accuracy. The area values under the curve of lncRNA were 0.8052(LNCV6_116109)、0.7088(LNCV6_98390)、0.7460(LNCV6_38772)、0.7292(LNCV6_108266)、0.7356(LNCV6_84003)and 0.6800(LNCV6_98602. Conclusion: This study showed that the expression of exosome LncRNAs (LNCV6_84003, LNCV6_98602, LNCV6_108266, LNCV6_116109, LNCV6_98390 and LNCV6_38772) was significantly up-regulated in plasma of patients with AS. They may serve AS potential non-invasive biomarkers for early diagnosis of AS.
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2023, Vol. 29 
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