Abstract：Objective: To investigate the impact of baicalin (Bai) on the malignant biological behavior of tongue cancer cells by regulating Notch/JAG1 signal pathway. Methods: Human oral epithelial cells HOEC was treated with 0, 10, 20, 30, 40, 50 and 100 μM Bai. Human tongue phosphorous carcinoma cell line CAL27 was grouped into CAL27 group (untreated CAL27 cells), L-Bai group (10μM Bai treated CAL27 cells), M-Bai group (20μM Bai treated CAL27 cells), H-Bai group (40μM Bai treated CAL27 cells), VPA group (8 mM Notch/JAG1 pathway activator VPA treated CAL27 cells), and H-Bai+VPA group (40μM Bai and 8 mM VPA co-treated CAL27 cells); MTT assay was applied to detect the effects of Bai on HOEC cytotoxicity and CAL27 cell viability; the apoptosis of CAL27 cells was detected by flow cytometry; Transwell was applied to detect the invasion and migration abilities of CAL27 cells; Western blot was applied to detect the expression of epithelial-mesenchymal transformation (EMT) protein and Notch/JAG1 signaling pathway protein. Results: 0~100μM Bai had no obvious toxic effect on HOEC cells. Compared with CAL27 group, the OD value, cell migration number, invasion number, the protein levels of vimentin, Snail, the protein levels of Notch1 and JAG1 in L-Bai group, M-Bai group and H-Bai group decreased obviously in turn (P<0.05), the apoptosis rate and the level of E-cadherin protein increased obviously in turn (P<0.05), in the VPA group, the above indicators had the opposite trends; compared with H-Bai group, the OD value, cell migration number, invasion number, the protein levels of vimentin, Snail, the protein levels of Notch1 and JAG1 in H-Bai+VPA group increased obviously (P<0.05), the apoptosis rate and the level of E-cadherin protein decreased obviously (P<0.05). Conclusion: Bai may affect the proliferation, apoptosis and EMT of tongue cancer cells by inhibiting Notch/JAG1 signaling pathway.
王欢, 夏力, 李玮, 谢春凤. 黄芩苷调节Notch/JAG1信号通路对舌癌细胞恶性生物学行为的影响[J]. 河北医学, 2023, 29(9): 1438-1443.
WANG Huan, XIA Li, LI Wei, et al. Impact of Baicalin on Malignant Biological Behavior of Tongue Cancer Cells by Regulating Notch/JAG1 Signaling Pathway. HeBei Med, 2023, 29(9): 1438-1443.
[1] Zhong W,Xu Z,Wen S,et al.Long non-coding RNA myocardial infarction associated transcript promotes epithelial-mesenchymal transition and is an independent risk factor for poor prognosis of tongue squamous cell carcinoma[J].Oral Pathol Med,2019,48(8):720-727. [2] Nicolini A,Ferrari P,Duffy MJ.Prognostic and predictive biomarkers in breast cancer:past,present and future[J].Semin Cancer Biol,2018,52(1):56-73. [3] Li J,Liu H,Lin Q,et al.Baicalin suppresses the migration and invasion of breast cancer cells via the TGF-β/lncRNA-MALAT1/miR-200c signaling pathway[J].Medicine (Baltimore),2022,101(46):29328-29334. [4] Zhang TH,Liang LZ,Liu XL,et al.LncRNA UCA1/miR-124 axis modulates TGFβ1-induced epithelial-mesenchymal transition and invasion of tongue cancer cells through JAG1/Notch signaling[J].Cell Biochem,2019,120(6):10495-10504. [5] Dou Y,Shang Y,Shen Y,et al.Baicalin alleviates adriamycin-induced focal segmental glomerulosclerosis and proteinuria by inhibiting the Notch1-Snail axis mediated podocyte EMT[J].Life Sci,2020,257(1):118010-118023. [6] Wang Z,Ma L,Su M,et al.Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling[J].Cell Death Dis,2018,9(2):217-33. [7] Yang X,Liu J,Liang Q,et al.Valproic acid reverses sorafenib resistance through inhibiting activated Notch/Akt signaling pathway in hepatocellular carcinoma[J].Fundam Clin Pharmacol,2021,35(4):690-699. [8] Yang B,Bai H,Sa Y,et al.Inhibiting EMT,stemness and cell cycle involved in baicalin-induced growth inhibition and apoptosis in colorectal cancer cells[J].Cancer,2020,11(8):2303-2317. [9] Fedele M,Sgarra R,Battista S,et al.The epithelial-mesenchymal transition at the crossroads between metabolism and tumor progression[J].Int Mol Sci,2022,23(2):800-833. [10] Hoshiba T.An extracellular matrix (ECM) model at high malignant colorectal tumor increases chondroitin sulfate chains to promote epithelial-mesenchymal transition and chemoresistance acquisition[J].Exp Cell Res,2018,370(2):571-578. [11] Tchekneva EE,Goruganthu MUL,Uzhachenko RV,et al.Determinant roles of dendritic cell-expressed notch delta-like and jagged ligands on anti-tumor T cell immunity[J].Immunother Cancer,2019,7(1):95-111. [12] Zhang Y,Aodeng G,Liu P,et al.Effects of HOX transcript antisense intergenic RNA on the metastasis,epithelial-mesenchymal transition and Notch signaling pathway in tongue cancer[J].Transl Cancer Res,2021,10(1):520-528. [13] Liu F,Chu HX,Han JS,et al.Inhibitory effect of the Notch pathway-inhibitor DAPT on invasion and metastasis of tongue cancer via lncRNA-KAT14 regulation[J].Eur Rev Med Pharmacol Sci,2020,24(1):189-199.
2023, Vol. 29 
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