Abstract：Objective: To investigate the diagnostic significance of hTERC RNA fractions expression detected by RNA scope technique in cervical squamous intraepithelial neoplasia. Methods: A total of 80 cervical biopsy specimens diagnosed with HR-HPV infection in the Department of Pathology, Affiliated Hospital of North China University of Science and Technology from September 2021 to February 2022 were screened in 3 groups: inflammation group (14 cases), low-grade group (34 cases), and high-grade group (32 cases). The amplification status of hTERC gene and the expression of its RNA components in paraffin-embedded tissues were detected by fluorescence in situ hybridization and RNAscope techniques, respectively. Results: FISH detection of hTERC gene amplification was different among the three groups (P<0.05). Detection rate by RNAscope was statistically different among the three groups (P<0.05). In the histological diagnostic subgroups, the degree of positive expression of hTERC RNA fraction was (-/++) in all 14 cases in the inflammation group, the hTERC RNA fraction (++) was predominant in the low-grade group (19/34), and the hTERC RNA fraction (++++/++++) was predominant in the high-grade group (27/32), and the expression of the above staining patterns in the histological graded diagnosis had statistical differences (P<0.05). Conclusion: The RNAscope technique to detect the expression of hTERC RNA fraction in cervical intraepithelial neoplasia tissue helps in the histological diagnosis and progression prediction of cervical lesions.
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2023, Vol. 29 
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