卵巢癌组织中circKIF4A和miR-942-5p的表达及其与患者临床病理特征及预后的关系

doi:10.3969/j.issn.1006-6233.2023.01.024

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摘要 目的: 探讨miR-942-5p、circKIF4A在卵巢癌组织中的表达及与患者预后的关系。方法: 选取2015年1月至2017年1月来本院进行手术治疗的154例卵巢癌患者为研究对象,收集手术患者的癌组织及癌旁组织,回顾性分析miR-942-5p、circKIF4A表达与临床病理特征及与预后的关系。实时荧光定量PCR(qRT-PCR)法检测miR-942-5p、circKIF4A水平;利用Kaplan-Meier法分析不同miR-942-5p、circKIF4A表达与患者生存时间的关系;多因素Cox回归风险模型分析影响患者预后的因素。结果: circKIF4A在卵巢癌患者癌组织中的表达(4.27±0.33)较癌旁组织(1.00±0.05)升高,miR-942-5p表达(0.63±0.12)较癌旁组织(1.01±0.19)降低,二者表达呈负相关(r=-0.312,P<0.05);卵巢癌患者癌组织中circKIF4A、miR-942-5p表达与年龄、病理类型、WHO组织学分级、肿瘤直径、浸润程度无关,与分化程度、淋巴结转移、TNM分期、肿瘤分级、有无腹水有关(P<0.05);Kaplan-Meier分析结果显示,circKIF4A高表达组患者5年累积生存率26.92%(21/78)显著低于低表达组47.37%(36/76),差异有统计学意义(Log rank χ²=6.651,P=0.010);miR-942-5p高表达组患者5年累积生存率46.25%(37/80)显著高于低表达组患者27.03%(20/74),差异有统计学意义(Log rank χ²=9.978,P=0.000);Cox结果显示,淋巴结转移、TNM分期、分化程度、circKIF4A高表达、miR-942-5p低表达均是影响卵巢癌患者生存状况的危险因素。结论: 卵巢癌患者癌组织中miR-942-5p呈低表达,circKIF4A呈高表达,二者表达呈负相关,与卵巢癌患者病情发展及预后关系密切。

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关键词 ：卵巢癌, circKIF4A, miR-942-5p, 病理特征, 预后

Abstract：Objective: To investigate the expressions of miR-942-5p and circKIF4A in ovarian cancer tissues and their relationship with the prognosis of patients. Methods: A total of 154 ovarian cancer patients who came to our hospital for surgical treatment from January 2015 to January 2017 were collected as the research subjects. The cancer tissues and adjacent tissues of the surgical patients were collected, and the relationship between the expressions of miR-942-5p and circKIF4A with clinicopathological characteristics and prognosis was retrospectively analyzed. The levels of Mir-942-5p and circKIF4A were detected by real-time fluorescence quantitative PCR (QRT-PCR); Kaplan-Meier method was used to analyze the relationship between the expression of different miR-942-5p and circKIF4A and the survival time of patients; multivariate Cox regression risk model was used to analyze the prognostic factors. Results: The expression of circKIF4A was higher in cancer tissues of ovarian cancer patients (4.27±0.33) compared with paraneoplastic tissues (1.00±0.05), and miR-942-5p expression was lower (0.63±0.12) compared with paraneoplastic tissues (1.01±0.19), with a negative correlation between the two expressions (r=-0.312, P<0.05); CircKIF4A and miR-942-5p expression in cancer tissues of ovarian cancer patients was not associated with age, pathological type, WHO histological grade, tumor diameter and degree of infiltration, but with degree of differentiation, lymph node metastasis, TNM stage, tumor grade and presence of ascites (P<0.05); Kaplan-Meier analysis showed that the 5-year cumulative survival rate of patients in the circKIF4A high expression group was significantly lower at 26.92% (21/78) than that of patients in the low expression group at 47.37% (36/76), with a statistically significant difference (Log rank χ²=6.651, P=0.010); The 5-year cumulative survival rate of patients in the miR-942-5p high expression group was significantly higher at 46.25% (37/80) than that of patients in the low expression group at 27.03% (20/74), with a statistically significant difference (Log rank χ²=9.978, P=0.000); Cox results showed that lymph node metastasis, TNM stage, degree of differentiation, high expression of circKIF4A, and miR-942-5p low expression were all risk factors affecting the survival status of ovarian cancer patients. Conclusion: The expression of miR-942-5p was low and circKIF4A was high in cancer tissues of ovarian cancer patients, and the expression of both was negatively correlated with the disease development and prognosis of ovarian cancer patients.

Key words： Ovarian cancer CircKIF4A miR-942-5p Pathological features Prognosis

基金资助:湖北省自然科学基金,(编号:WJ2017S006)

通讯作者: 喻欣

引用本文:

汤明, 方春田, 王燕, 张仙, 喻欣. 卵巢癌组织中circKIF4A和miR-942-5p的表达及其与患者临床病理特征及预后的关系[J]. 河北医学, 2023, 29(1): 126-131.
TANG Ming, FANG Chuntian, et al. Expression of CircKIF4A and miR-942-5p in Ovarian Cancer Tissues and Their Relationship with Clinicopathological Characteristics and Prognosis of Patients. HeBei Med, 2023, 29(1): 126-131.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2023.01.024 或 http://www.hbyxzzs.cn/CN/Y2023/V29/I1/126

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