白芦藜醇对大鼠牙移植后牙周组织愈合及Sirt1/Nrf2信号通路的影响

doi:10.3969/j.issn.1006-6233.2022.11.06

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摘要 目的: 观察白芦藜醇(RSV)对大鼠牙移植后牙周组织愈合及Sirt1/Nrf2信号通路的影响。方法: 48只雄性Lewis大鼠随机分为对照(Control)组、模型(Model)组、低剂量RSV(L-RSV)组和高剂量RSV(H-RSV)组。苏木精-伊红(HE)染色、抗酒石酸酸性磷酸酶(TRAP)染色观察牙周组织病理学变化;免疫组织化学染色检测核因子κB受体激活剂配体(RANKL)、骨保护素(OPG)、Runt相关转录因子2(RUNX2)和骨钙素(OCN)等成骨分化相关因子的表达;扫描电子显微镜分析牙根吸收情况,Western blot检测牙周组织中Sirt1、Nrf2蛋白表达水平。结果: 与Control组比较,Model组牙周组织TRAP阳性细胞数量增多,RANKL表达和牙根吸收率升高,OPG、RUNX2和OCN表达以及Sirt1、Nrf2蛋白水平降低(P<0.05)。与Model组比较,L-RSV组和H-RSV组牙周组织TRAP阳性细胞数量减少;RANKL表达和牙根吸收率降低,OPG、RUNX2和OCN表达以及Sirt1、Nrf2蛋白水平升高(P<0.05)。结论: RSV可促进大鼠牙移植后牙周组织愈合,促进作用可能与激活Sirt1/Nrf2信号通路有关。

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关键词 ：白芦藜醇, 大鼠, 牙移植, 牙周组织, Sirt1/Nrf2信号通路

Abstract：Objective: To observe the influences of resveratrol (RSV) on the periodontal tissue healing and Sirt1/Nrf2 signaling pathway after tooth transplantation in rats.Methods: 48 male Lewis rats were randomly divided into control (Control) group, model (Model) group, low-dose RSV (L-RSV) group, and high-dose RSV (H-RSV) group. Hematoxylin-eosin (HE) staining and tartrate-resistant acid phosphatase (TRAP) staining were used to observe the pathological changes in periodontal tissues. Immunohistochemical staining was used to detect nuclear factors κB receptor activator ligand (RANKL), osteoprotegerin (OPG), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and other osteogenic differentiation-related factors. The root resorption was analyzed by scanning electron microscope. The expression levels of SIRT1 and Nrf2 proteins in periodontal tissue were detected by Western blot. Results: Compared with the control group, the number of TRAP-positive cells in the periodontal tissue of the model group increased; the expression of RANKL was increased, and the expressions of OPG, RUNX2, and OCN were decreased; the root resorption rate was increased; the protein levels of Sirt1 and Nrf2 were decreased (P<0.05). Compared with the model group, the number of TRAP-positive osteoclasts in the periodontal tissue of the L-RSV group and the H-RSV group decreased; the expression of RANKL was decreased, and the expressions of OPG, RUNX2, and OCN were increased; the root resorption rate was significantly decreased; Sirt1, Nrf2 protein levels increased (P<0.05). Conclusion: RSV can promote the healing of periodontal tissue after tooth transplantation in rats, and the promotion may be related to the activation of the Sirt1/Nrf2 signaling pathway.

Key words： Resveratrol Rat Tooth transplantation Periodontal tissue Sirt1/Nrf2 signaling pathway

基金资助:湖北省武汉市卫生和计划生育委员会科研项目,(编号:WX18C06)

通讯作者: 陈慧

引用本文:

王鹏, 陈慧, 代喆颖, 江铃, 彭红, 周辉. 白芦藜醇对大鼠牙移植后牙周组织愈合及Sirt1/Nrf2信号通路的影响[J]. 河北医学, 2022, 28(11): 1790-1794.
WANG Peng, CHEN Hui, DAI Zheying, et al. Influences of Resveratrol on Periodontal Tissue Healing and Sirt1/Nrf2 Signaling Pathway after Tooth Transplantation in Rats. HeBei Med, 2022, 28(11): 1790-1794.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.11.06 或 http://www.hbyxzzs.cn/CN/Y2022/V28/I11/1790

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