Abstract：Objective: To investigate the effects of midazolam (MID) on the proliferation and apoptosis of esophageal cancer cells by regulating the miR-875-5p/stromal antigen 2 (STAG2) axis. Methods: Esophageal cancer Eca109 cells were treated with different concentrations of MID (0,10,20,30,40 μmoL/L) for 24h,and the appropriate MID concentration was screened by CCK-8 method; high-throughput miRNA transcriptomics was used to screen the differentially expressed miRNAs in 30 μmoL/L-treated Eca109 cells; miR-875-5p and STAG2 mRNA expression levels were examined by qRT-PCR; the relationship between miR-875-5p and STAG2 was verified by dual luciferase assay; miR-875-5p mimics (miR-875-5p mimics) were transfected in Eca109 cells and intervened with 30 μmoL/L MID to obeserve survival rate,apoptosis rate and cell cycle changes;Western blot was used to detect the expression level of STAG2 protein;in vivo xenograft tumor experiments were conducted to detect the effects of MID and miR-875-5p on the growth of xenograft tumors in nude mice. Results: 30μmoL/L MID was used for follow-up research; miR-875-5p was screened as significantly down-regulated miRNAs in Eca109 cells treated with 30μmoL/L; 30μmoL/L MID intervention or down-regulation of miR-875-5p expression could reduce the proliferation ability,miR-875-5p expression,and the proportion of cells in S phase of Eca109 cells,while the apoptotic rate and the proportion of cells in G0/G1 phase increased (P<0.05 ); there is a negative regulatory relationship between miR-875-5p and STAG2; up-regulating the expression of miR-875-5p can reverse the effects of MID on the proliferation,apoptosis and cell cycle of Eca109 cells; at the same time,it can also reverse the inhibitory effect of MID on the growth of transplanted tumors in nude mice. Conclusion: MID targets and down-regulates the level of miR-875-5p to promote the expression of STAG2,thereby inhibiting the proliferation and promoting cell apoptosis of Eca109 cells.
刘梦诗, 崔光锐, 邱坚, 廖微微. 咪达唑仑调控miR-875-5p/STAG2轴对食管癌细胞增殖及凋亡的影响[J]. 河北医学, 2022, 28(10): 1585-1590.
LIU Mengshi, CUI Guangrui, QIU Jian, et al. Effect of Midazolam Regulation of miR-875-5p/STAG2 Axis on Proliferation and Apoptosis of Esophageal Cancer Cells. HeBei Med, 2022, 28(10): 1585-1590.
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2022, Vol. 28 
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