Nckα基因沉默对上皮性卵巢癌细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-6233.2022.07.03

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摘要 目的: 探讨Nckα基因沉默对上皮性卵巢癌细胞恶性生物学行为的影响。方法: 选取正常卵巢组织切片、良性卵巢病变组织切片以及卵巢癌组织切片分析Nckα表达特点,通过免疫荧光、细胞侵袭、迁移实验、细胞增殖实验、构建皮下移植瘤动物模型以及Western blot检测等方式检测Nckα沉默对卵巢癌SKOV3细胞株、OVCAR-3细胞株的影响。结果: Nckα基因沉默可降低卵巢癌细胞侵袭、迁移能力;Nckα沉默卵巢癌SKOV3细胞、OVCAR3细胞吸光度值明显低于对照组,Nckα过表达卵巢癌SKOV3细胞、OVCAR3细胞吸光度值明显高于对照组(P<0.05);Nckα沉默组细胞瘤体体积小于对照组(P<0.05);Nckα沉默卵巢癌SKOV3细胞、OVCAR3细胞PI3K p110α、p70S6K、AKT、P-AKT蛋白表达明显低于对照组,Nckα过表达卵巢癌SKOV3细胞、OVCAR3细胞PI3K p110α、p70S6K、AKT、P-AKT蛋白表达明显高于对照组(P<0.05)。结论: Nckα基因沉默可导致上皮性卵巢癌细胞增殖、迁移能力降低,延缓皮下移植瘤生长速度,可能与Nckα基因沉默调控PI3K p110α、p70S6K、AKT、P-AKT蛋白表达有关。

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关键词 ： Nckα基因, 卵巢癌, 上皮性病变

Abstract：Objective: To investigate the effect of Nckα gene silencing on the malignant biological behavior of epithelial ovarian cancer cells. Methods: The expression characteristics of Nckα were analyzed in normal ovarian tissue sections,benign ovarian tissue sections and ovarian cancer tissue sections.The effects of Nckα silencing on ovarian cancer cell lines SKOV3 and OVCAR-3 were detected by immunofluorescence,cell invasion,migration,cell proliferation,subcutaneous xenograft animal model,and Western blot. Results: Nckα gene silencing could reduce the invasion and migration ability of ovarian cancer cells.The absorbance of SKOV3 and OVCAR3 cells with Nckα silence was significantly lower than that of the control group,and the absorbance of SKOV3 and OVCAR3 cells with Nckα overexpression was significantly higher than that of the control group (P<0.05).The tumor volume of Nckα silenced group was lower than that of control group (P<0.05).The protein expressions of PI3K P110 α,p70S6K,AKT and P-Akt in SKOV3 cells and OVCAR3 cells silenced by Nckα were significantly lower than those in the control group.The protein expressions of PI3K P110 α,p70S6K,AKT and P-Akt in SKOV3 cells and OVCAR3 cells with Nckα overexpression were significantly higher than those in the control group (P<0.05). Conclusion: Nckα gene silencing can reduce the proliferation and migration ability of epithelial ovarian cancer cells,and delay the growth rate of subcutaneous graft tumor,which may be related to the regulation of PI3K P110 α,p70S6K,AKT and P-Akt protein expression by Nckα gene silencing.

Key words： Nckα gene Ovarian cancer Epithelial lesions

基金资助:海南省自然科学基金项目,(编号:817362);2.重庆市科技惠民计划项目,(编号:cstc2016k jhmpt1001-3)

通讯作者: 高天

引用本文:

周颖, 高天, 杜颖. Nckα基因沉默对上皮性卵巢癌细胞恶性生物学行为的影响[J]. 河北医学, 2022, 28(7): 1066-1071.
ZHOU Ying, et al. Effect of Nckα Gene Silencing on Malignant Biological Behavior of Epithelial Ovarian Cancer Cells. HeBei Med, 2022, 28(7): 1066-1071.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.07.03 或 http://www.hbyxzzs.cn/CN/Y2022/V28/I7/1066

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