Abstract：Objective: To study the role of lncRNA CYTOR on the proliferation, migration and invasion in oral squamous cell carcinoma (OSCC) and its underlying molecular regulatory mechanisms. Methods: CAL27 cells at logarithmic phase were randomly divided into Control group (normal cultured cells), si-NC group (transfected with empty plasmid) and si-lncRNA CYTOR group (transfected with si-lncRNA CYTOR plasmid). RT-qPCR was used to detect the expression of lncRNA CYTOR in normal oral cells (NHOK) and OSCC cells (Tca8113, CAL27, SCC9 and SCC25). Cell proliferation was detected by CKK-8, clone formation and EdU staining assays, cell migration and invasion were detected by Transwell assay, and the expression of cell proliferation-related proteins (PNCA and Ki-67) and Wnt/β-catenin pathway-related proteins (c-myc, cyclin D1 and β-catenin) were detected by Western blot. Results: Compared with NHOK cells, the expression of lncRNA CYTOR in OSCC cells (Tca8113, CAL27, SCC9 and SCC25) was significantly decreased, especially in CAL27 cells (P<0.01). The evaluation of cell function in vitro showed that compared with si-NC group, cell activity, clone formation ability, the proportion of EdU positive cells, migration and invasion in si-lncRNA CYTOR group were significantly reduced (P<0.01). In addition, compared with si-NC group, the expression levels of PCNA, Ki-67, c-myc, cyclin D1 and β-catenin in si-lncRNA CYTOR group were significantly decreased (P<0.01). Conclusion: LncRNA CYTOR is highly expressed in OSCC, and lncRNA CYTOR silencing could suppress the proliferation, migration and invasion of CAL27 cells, and its mechanism may be related to the inhibition of Wnt/β-catenin pathway.
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2022, Vol. 28 
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