β<sub>2</sub>-MG miR-182 BUN在老年慢性肾功能衰竭中的表达及相关性研究

doi:10.3969/j.issn.1006-6233.2022.05.011

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摘要 目的:研究β₂微球蛋白(β₂-microglobulin,β₂-MG)、微小RNA-182(miR-182)、血尿素氮(Blood ureanitrogen,BUN)在老年慢性肾功能衰竭中的表达及相关性。方法:选择2019年3月至2020年12月接受治疗的慢性肾功能衰竭患者43例,作为研究组;选取于同时期进行健康体检的正常人43例,作为对照组。血清miR-182表达采用荧光定量聚合酶链式反应技术检测,采用免疫比浊法检测β₂-MG、BUN水平,采用酶联免疫吸附测定法检测血清CYSC、Scr、RBP水平,受试者特征曲线(receiver operator characteristics curve,ROC)分析β₂-MG、miR-182、BUN在老年慢性肾功能衰竭中诊断价值。结果:研究组β₂-MG、miR-182、BUN、CYSC、Scr、RBP表达水平均高于对照组(P<0.05)。相关性分析显示,β₂-MG与miR-182之间呈正相关(r=0.695,P=0.001);β₂-MG与BUN之间呈正相关(r=0.733,P=0.001);miR-182与BUN之间呈现正相关(r=0.778,P=0.001)(P<0.05)。研究组早期患者β₂-MG、miR-182、BUN水平高于非早期患者(P<0.05)。年龄≥65岁、少尿、水肿、大量尿蛋白老年慢性肾功能衰竭患者β₂-MG、miR-182、BUN表达水平较高(P<0.05)。ROC分析β₂-MG、miR-182、BUN诊断老年慢性肾功能衰竭灵敏度低于三项联合(P<0.05)。结论:β₂-MG、miR-182、BUN在老年慢性肾功能衰竭中高表达,且三者之间具有相关性,在老年慢性肾功能衰竭的诊断中具有一定的临床价值。

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	孙瑶
	孙琨

关键词 ：老年慢性肾功能衰竭, β₂微球蛋白, 微小RNA-182, 血尿素氮

Abstract：Objective: To study the expression and correlation of β₂ microglobulin (β₂-microglobulin, β₂-MG), microRNA-182 (miR-182), and blood ureanitrogen (BUN) in elderly chronic renal failure. Methods: Totally 43 patients with chronic renal failure treated from March 2019 to December 2020 were selected as the study group; 43 normal subjects who underwent physical examination in the same period were selected as the control group. Serum miR-182 expression was detected by fluorescence quantitative polymerase chain reaction technique, β₂-MG and BUN levels were detected by immunoturbidimetric method, serum CYSC, Scr and RBP levels were detected by enzyme-linked immunosorbent assay, and subject operator characteristics curve (ROC) was used to analyze the diagnostic value of β₂-MG, miR-182 and BUN in chronic renal failure in the elderly. Results: The expression levels of β₂-MG, miR-182, and BUN in the study group were higher than those in the control group (P<0.05). Correlation analysis showed a positive correlation between β₂-MG and miR-182 (r=0.695, P=0.001); between β₂-MG and BUN (r=0.733, P=0.001); and between miR-182 and BUN (r=0.778, P=0.001) (P<0.05). The levels of β₂-MG, miR-182 and BUN were higher in early stage patients than in non-early stage patients in the study group (P<0.05). The expression levels of β₂-MG, miR-182 and BUN were higher in elderly patients with chronic renal failure aged ≥65 years, with oliguria, oedema and large amounts of urine protein (P<0.05). ROC analysis of β₂-MG, miR-182 and BUN was less sensitive than the three combined in diagnosing elderly chronic renal failure (P<0.05). Conclusion: β₂-MG, miR-182, and BUN are highly expressed in elderly chronic renal failure, and there is a correlation between the three, which has certain clinical value in the diagnosis of elderly chronic renal failure.

Key words： Elderly chronic renal failure β₂ microglobulin MicroRNA-182 Blood urea nitrogen

基金资助:山东省卫生厅指导性科研计划项目,(编号:SG20200103)

通讯作者: 孙琨

引用本文:

孙瑶, 孙琨. β₂-MG miR-182 BUN在老年慢性肾功能衰竭中的表达及相关性研究[J]. 河北医学, 2022, 28(5): 754-758.
SUN Yao, SUN Kun. Expression and Correlation Studies of β₂-MG MiR-182 BUN in Elderly Chronic Renal Failure. HeBei Med, 2022, 28(5): 754-758.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.05.011 或 http://www.hbyxzzs.cn/CN/Y2022/V28/I5/754