七氟醚基于BDNF/TrkB信号通路对孕晚期大鼠子代树突棘发育的影响

doi:10.3969/j.issn.1006-6233.2022.04.08

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摘要 目的:探究七氟醚麻醉对孕晚期大鼠子代树突棘发育的影响及其机制。方法:取孕大鼠分为对照组,七氟醚-1h组,七氟醚-3h组,七氟醚-6h组,以2.3%七氟醚分别麻醉。取P31子代幼鼠开展水迷宫测试,以高浓度CO₂处死,取脑组织开展电生理实验检测海马CA1区长时程增强,高尔基染色评估树突棘状态,Western blot检测BDNF/TrkB蛋白表达。结果:与对照组相比,七氟醚麻醉1h的孕晚期大鼠子代P35幼鼠水迷宫实验中逃避潜伏期没有改变,而七氟醚-3h组与七氟醚-6h组幼鼠逃避潜伏期明显增加,穿越平台次数与第Ⅲ象限停留时间明显减少。此外,七氟醚麻醉3h与6h造成幼鼠fEPSP斜率明显降低,且树突棘密度与长度明显减少,然而,七氟醚-1h组幼鼠fEPSP与树突棘状态没有影响。与此同时,七氟醚麻醉3h与6h造成了幼鼠海马组织中PSD-95,BDNF与p-TrkB表达明显减少。结论:孕晚期大鼠长期麻醉后幼鼠的学习与记忆能力降低是由BDNF表达降低引起,其原因BDNF造成p-TrkB表达降低介导PSD-95蛋白减少,导致树突棘发育受损。

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关键词 ：七氟醚, 孕晚期大鼠子代, 树突棘, 脑源性神经营养因子

Abstract：Objective: To explore the effect of sevoflurane anesthesia on dendritic spine development in late pregnant rat offspring and its mechanism. Methods: Pregnant rats were divided into control group, sevoflurane-1h group, sevoflurane-3h group and sevoflurane-6h group, they were anesthetized with 2.3% sevoflurane respectively. P31 offspring rats were subjected to water maze test and executed with high CO₂ concentration. Brain tissue was taken for electrophysiological experiments to detect long duration enhancement in CA1 region of hippocampus, Golgi staining to assess dendritic spine status and western blot to detect BDNF/TrkB protein expression.Results: Compared to the control group, the latency to evade was not altered in the water maze experiment in late pregnant rat offspring P35 pups anesthetized with sevoflurane for 1h, whereas the latency to evade increased significantly in the sevoflurane-3h and sevoflurane-6h groups, and the number of platform crossings and quadrant III dwell time decreased significantly. In addition, sevoflurane anesthesia at 3h and 6h caused a significant decrease in the slope of fEPSP and a significant decrease in the density and length of dendritic spines in the pups, however, there was no effect on the fEPSP and dendritic spine status in the sevoflurane-1h group. At the same time, the expression of PSD-95, BDNF and p-TrkB in hippocampal tissues of young rats was significantly reduced by 3h and 6h sevoflurane anesthesia.Conclusion: The reduced learning and memory capacity in late pregnant rat pups after prolonged anaesthesia is caused by reduced BDNF expression, which results from reduced p-TrkB expression mediating reduced PSD-95 protein, leading to impaired dendritic spine development.

Key words： Sevoflurane Offspring of the third trimester pregnant rats Dendritic spines Brain-derived neurotrophic factor

基金资助:吴阶平医学基金会临床科研专项资助基金,(320.6750.2021-01-05)

引用本文:

苏娟, 张振忠, 张友佳. 七氟醚基于BDNF/TrkB信号通路对孕晚期大鼠子代树突棘发育的影响[J]. 河北医学, 2022, 28(4): 567-571.
SU Juan, et al. Effect of Sevoflurane Based on BDNF/TrkB Signaling Pathway on Dendritic Spine Development in Rat Offspring During Late Pregnancy. HeBei Med, 2022, 28(4): 567-571.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.04.08 或 http://www.hbyxzzs.cn/CN/Y2022/V28/I4/567

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