阿尔茨海默病患者血清miR-137 ABCA7水平变化及临床意义

doi:10.3969/j.issn.1006-6233.2021.10.014

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摘要 目的:探讨阿尔茨海默病(AD)患者血清微小RNA(miRNA)-137、三磷酸腺苷结合盒转运蛋白7(ABCA7)水平变化及临床意义。方法:选取我院2019年1月至2020年12月收治的138例AD患者为AD组,根据临床痴呆评定量表(CDR)分为轻度组(n=48)、中度组(n=59)、重度组(n=31),另选取同期64名体检健康者为对照组。比较两组血清miR-137、ABCA7、β-淀粉样蛋白42(Aβ42)、Aβ40、Aβ42/Aβ40、血脂水平和简易精神状态量表(MMSE)评分。Spearman相关性分析AD组血清miR-137、ABCA7水平与血清Aβ42、Aβ40、Aβ42/Aβ40、血脂水平和MMSE评分的相关性。ROC曲线分析血清miR-137、ABCA7水平对AD的诊断价值。结果:AD组血清miR-137、Aβ42、高密度脂蛋白胆固醇(HDL-C)水平和Aβ42/Aβ40、MMSE评分明显低于对照组,ABCA7、Aβ40、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(LDL-C)水平明显高于对照组(P<0.05)。Spearman相关性分析显示,AD组血清miR-137水平与Aβ42、Aβ42/Aβ40、HDL-C和MMSE评分呈正相关,与Aβ40、TC、LDL-C水平、CDR评分呈负相关(P<0.05);血清ABCA7水平与Aβ42、Aβ42/Aβ40、HDL-C和MMSE评分呈负相关,与Aβ40、TC、LDL-C水平、CDR评分呈正相关(P<0.05)。ROC曲线显示,miR-137+ABCA7诊断AD的曲线下面积(AUC=0.925)明显大于miR-137(AUC=0.837)、ABCA7(AUC=0.823)单独诊断(P<0.05),敏感度、特异度为85.51%、84.37%。结论:AD患者血清miR-137水平明显降低,ABCA7水平明显提高,与AD患者Aβ沉积、胆固醇代谢异常、病情密切相关,可能成为AD诊断、病情严重程度评估标志物,二者联合检测价值更高。

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关键词 ：阿尔茨海默病, 微小RNA-137, 三磷酸腺苷结合盒转运蛋白7, β-淀粉样蛋白, 胆固醇

Abstract：Objective: To investigate the changes and clinical significance of serum microRNA (miRNA)-137 and adenosine triphosphate binding cassette transporter 7 (ABCA7) levels in patients with Alzheimer's disease (AD). Methods: A total of 138 AD patients admitted to our hospital from January 2018 to December 2019 were selected as the AD group. According to the clinical dementia rating (CDR) scale, they were divided into mild group (n=48) and moderate group (n=59), severe group (n=31), and 64 healthy people during physical examination during the same period were selected as the control group. Spearman correlation analysis was conducted to analyze the correlation between AD group's serum miR-137, ABCA7 levels and serum Aβ42, Aβ40, Aβ42/Aβ40, blood lipid levels and MMSE score. ROC curve was plotted to analyze serum miR-137 and ABCA7 levels in the diagnosis of AD. Results: The serum miR-137, Aβ42, high-density lipoprotein cholesterol (HDL-C) levels, Aβ42/Aβ40 and MMSE scores in the AD group were significantly lower than those in the control group, and ABCA7, Aβ40, total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels were significantly higher than those of the control group (P<0.05). Spearman correlation analysis showed that serum miR-137 levels in AD group were positively correlated with Aβ42, Aβ42/Aβ40, HDL-C and MMSE scores, and negatively correlated with Aβ40, TC, LDL-C levels, and CDR scale (P<0.05); Serum ABCA7 levels were negatively correlated with Aβ42, Aβ42/Aβ40, HDL-C and MMSE scores, and positively correlated with Aβ40, TC, LDL-C levels, and CDR scale (P<0.05). The ROC curve showed that the area under the curve (AUC=0.925) of miR-137+ABCA7 for the diagnosis of AD was significantly greater than that of miR-137 (AUC=0.837), ABCA7 (AUC=0.823) in diagnosed alone (P<0.05), and the sensitivity and specificity were 85.51% and 84.37%. Conclusion: The level of serum miR-137 in AD patients is significantly reduced, and the level of ABCA7 is significantly increased. It is closely related to Aβ deposition, abnormal cholesterol metabolism, and disease condition in AD patients. It may be a marker for AD diagnosis and disease severity assessment. The combined detection of the two is more valuable.

Key words： Alzheimer's disease MicroRNA-137 Adenosine triphosphate binding cassette transporter 7 β-amyloid protein Cholesterol

基金资助:河北省医学科研课题,(编号:201801158)

通讯作者: 张凤莲

引用本文:

周育蕾, 陈艳洁, 刘泽民, 李艳琴, 段玉香, 张凤莲. 阿尔茨海默病患者血清miR-137 ABCA7水平变化及临床意义[J]. 河北医学, 2021, 27(10): 1652-1656.
ZHOU Yulei, CHEN Yanjie, LIU Zemin, et al. Changes of Serum MiR-137 and ABCA7 Levels in Patients with Alzheimer's Disease and Their Clinical Significance. HeBei Med, 2021, 27(10): 1652-1656.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.10.014 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I10/1652

[1] 中国痴呆与认知障碍诊治指南写作组,中国医师协会神经内科医师分会认知障碍疾病专业委员会.中国阿尔茨海默病一级预防指南[J].中华医学杂志,2020,100(35):2721~2735.
[2] 中华医学会放射学分会磁共振学组,北京认知神经科学学会.阿尔茨海默病MR检查规范中国专家共识[J].中华放射学杂志,2019,53(8):635~641.
[3] Swarbrick S,Wragg N,Ghosh S,et al.Systematic review of miRNA as biomarkers in alzheimer's disease[J].Mol Neurobiol,2019,56(9):6156~6167.
[4] Mahmoudi E,Cairns MJ.MiR-137:an important player in neural development and neoplastic transformation[J].Mol Psychiatry,2017,22(1):44~55.
[5] Loera-Valencia R,Goikolea J,Parrado-Fernandez C,et al.Alterations in cholesterol metabolism as a risk factor for developing Alzheimer's disease:potential novel targets for treatment[J].Steroid Biochem Mol Biol,2019,190:104~114.
[6] Aikawa T,Holm ML,Kanekiyo T.ABCA7 and pathogenic pathways of alzheimer's disease[J].Brain Sci,2018,8(2):27.
[7] American Psychiatric Association.Diagnostic and statistical manual of mental disorders.4th ed.(DSM-Ⅴ) [M].Washington DC:American Psychiatric Association,2013.
[8] 中国老年医学学会认知障碍分会.临床痴呆评定量表简体中文版[J].中华老年医学杂志,2018,37(4):367~371.
[9] Folstein MF,Folstein SE,McHugh PR."Mini-mental state".A practical method for grading the cognitive state of patients for the clinician[J].Psychiatr Res,1975,12(3):189~198.
[10] Geekiyanage H,Chan C.MicroRNA-137/181c regulates serine palmitoyltransferase and in turn amyloid β,novel targets in sporadic Alzheimer's disease[J].Neurosci,2011,31(41):14820~14830.
[11] Satoh K,Abe-Dohmae S,Yokoyama S,St George-Hyslop P,et al.ATP-binding cassette transporter A7 (ABCA7) loss of function alters Alzheimer amyloid processing[J].Biol Chem,2015,290(40):24152~24165.
[12] Lamartinière Y,Boucau MC,Dehouck L,et al.ABCA7 downregulation modifies cellular cholesterol homeostasis and decreases amyloid-β peptide efflux in an in vitro model of the blood-brain barrier[J].Alzheimers Dis,2018,64(4):1195~1211.