Abstract：Objective: To explore the expression levels of Adropin and zonula occludens-1 (ZO-1) in patients with hypertensive cerebral hemorrhage and its clinical significance. Methods: A total of 143 patients with hypertensive cerebral hemorrhage in our hospital from March 2017 to March 2020 were collected and divided into mild group (n=69, NIHSS score:1~14), moderate group (n=43, NIHSS score:15~20), and severe group (n=31, NIHSS score: 21-42) according to the degree of neurological deficit. The patients also were divided into good prognosis group (n=107, GOS score: 4~5) and poor prognosis group (n=36, GOS score: 1~3) according to the prognosis 6 months after treatment. At the same time, 71 healthy volunteers were randomly selected as control group. Serum Adropin and ZO-1 levels were detected by enzyme-linked immunosorbent assay. The receiver operating characteristic (ROC) curve was plotted to evaluate the predictive value of serum Adropin and ZO-1 for poor prognosis. Results: The serum Adropin level in case group was lower than that in control group [(1.53±0.41)μg/ml vs. (3.79±0.46)μg/ml; t=36.449], and ZO-1 level was higher than that in control group [(308.22±60.93)pg/ml vs. (149.86±51.04)pg/ml],the difference was statistically significant (P<0.05). The serum Adropin in severe group and moderate group was lower than that in mild group, and ZO-1 level was higher than that in mild group. Moreover, the serum Adropin in severe group was lower than that in moderate group, and ZO-1 level was higher than that in moderate group, the difference was statistically significant (P<0.05). The Adropin level in poor prognosis group was lower than that in good prognosis group, and ZO-1 level was higher than that in good prognosis group, the difference was statistically significant (P<0.05) . Pearson product moment correlation analysis showed that serum Adropin level was negatively associated with NIHSS score (r=-0.792, P<0.05), and positively associated with GOS score (r=0761, P<0.05). Serum ZO-1 level was positively associated with NIHSS score (r=0.708, P<0.05), and negatively associated with GOS score (r=-0.673, P<0.05). The result of ROC curve showed that serum Adropin and ZO-1 had predictive value for the poor prognosis of patients with hypertensive cerebral hemorrhage 6 months after treatment, the AUC was 0.790 and 0.761 respectively, and the Adropin combined with XO-1 had higher predictive value, the AUC was 0.837, the sensitivity and specificity were 83.04% and 78.65% respectively. The predictive value of combined detection was better than that of single index (P<0.05). Conclusion: The down-regulation of serum Adropin expression and up-regualtion of ZO-1 expression are involved in the process of hypertensive cerebral hemorrhage, which are closely related to the degree of neurological deficit and prognosis. Early detection of Adropin and ZO-1 can be used as important biochemical index to judge the severity of the disease and predict the prognosis.
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2021, Vol. 27 
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