Abstract：Objective: To investigate the effect of sRAGE on ovalbumin induced allergic rhinitis in mice and the effect of nitric oxide. Methods: 18 female Balb/c mice were divided into control group, allergic rhinitis and sRAGE intervention group. The model of allergic rhinitis was prepared. The mice were sensitized by intraperitoneal injection and nasal drops of ovalbumin. SRAGE, 100ug/ mouse was injected intraperitoneally before and 12 hours after the last time of OVP rhinoplasty. The expression of IL-4 and IFN - γ mRNA was detected by real-time fluorescent quantitative polymerase chain reaction (QRT PCR), and the protein expression of iNOS was detected by Western blot. Results: Compared with the control group, the number of pruritus was significantly increased, the mRNA level of IL-4 and the protein expression of iNOS were significantly increased, and the mRNA level of IFN - γ was decreased in the allergic rhinitis group; Compared with the allergic rhinitis group, the mRNA level of IL-4 and the protein expression of iNOS were significantly decreased, and the mRNA level of IFN - γ was increased. Conclusion: The changes of IL-4, IFN - γ and iNOS levels suggest that sRAGE may mediate the anti allergic effect of sRAGE.
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2021, Vol. 27 
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