Abstract：Objective: Circular RNA plays an important role in the occurrence and development of various human diseases. By detecting the expression of circHIPK3 in non-small cell lung cancer (NSCLC) and its correlation with clinicopathological characteristics and prognosis, the value of circHIPK3 as a biomarker of NSCLC for clinical diagnosis and decision-making was evaluated. Method: Using qRT-PCR to detect the expression level of circHIPK3 in 54 NSCLC cancer tissues and corresponding normal adjacent tissues to cancer, combined with clinical datas, the clinical value of circHIPK3 as a biomarker for diagnosis and prognosis of NSCLC was analyzed. Results: The expression level of circHIPK3 in cancer tissues of NSCLC patients was significantly increased compared with normal adjacent tissues adjacent to the cancer (P<0.001); the plasma circHIPK3 level of patients was significantly increased compared with the expression levels of healthy people (P<0.001). The expression of circHIPK3 in cancer tissues is related to tumor tissue differentiation, clinical stage, and lymph node metastasis. Receiver work curve analysis showed that circHIPK3 has relatively high diagnostic value.The area under the ROC curve of NSCLC cancer tissue was 0.890, and the area under the plasma ROC curve of NSCLC patients was 0.829. Kaplan-Meier analysis showed that circHIPK3 is closely related to the prognosis of patients, and patients with high expression have a lower survival rate(χ2=3.657,P=0.026). Cox multivariate regression analysis showed that high expression of circHIPK3 is an independent risk factor for poor prognosis of NSCLC. Conclusion: CircHIPK3 may be a potential molecular biomarker and therapeutic target for the diagnosis and prognosis of NSCLC.
[1] Bian L, Zhi X, Ma L, et al. Hsa-circRNA-103809 regulated the cell proliferation and migration in colorectal cancer via miR-532-3p / FOXO4 axis[J].Biochem Biophys Res Commun,2018,505(2):346~352. [2] Yang R, Xing L, Zheng X, et al. The circRNA circAGFG1 acts as a sponge of miR-195-5p to promote triple-negative breast cancer progression through regulating CCNE1 expression [J]. Molecular cancer, 2019, 18(1): 1~19. [3] Song T, Xu A, Zhang Z, et al. CircRNA hsa-circRNA-101996 increases cervical cancer proliferation and invasion through activating TPX2 expression by restraining miR-8075 [J]. Journal of cellular physiology, 2019, 234(8): 14296~14305. [4] Liu N, Zhang J, Zhang LY, et al. CircHIPK3 is upregulated and predicts a poor prognosis in epithelial ovarian cancer[J].Eur Rev Med Pharmacol Sci,2018,22(12):3713~3718. [5] Feng X Q, Nie S M, Huang J X, et al. Circular RNA circHIPK3 serves as a prognostic marker to promote chronic myeloid leukemia progression[J]. Neoplasma, 2020, 67(1): 171~177. [6] Qiu L, Huang Y, Li Z, et al. Circular RNA profiling identifies circADAMTS13 as a miR-484 sponge which suppresses cell proliferation in hepatocellular carcinoma[J].Mol Oncol,2019,13(2):441~455. [7] Zhang X, Xu Y, Yamaguchi K, et al. Circular RNA circVAPA knockdown suppresses colorectal cancer cell growth process by regulating miR-125a/CREB5 axis[J].Cancer Cell Int,2020,20(103):1~11. [8] Hu Y, Guo F, Zhu H, et al. Circular RNA-0001283 suppresses breast cancer proliferation and invasion via MiR-187/HIPK3 axis[J].Med Sci Monit,2020,20(118):1~12. [9] Tang X, Liu S, Ding Y, et al. Serum circular foxO3a serves as a novel prognostic biomarker in squamous cervical cancer[J].Cancer Manag Res,2020,12(25):31~40. [10] Xiao-Long M, Kun-Peng Z, Chun-Lin Z. Circular RNA circ-HIPK3 is down-regulated and suppresses cell proliferation, migration and invasion in osteosarcoma[J].Cancer,2018,9(10):1856~1862. [11] Ji W, Qiu C, Wang M, et al. Hsa-circ-0001649: A circular RNA and potential novel biomarker for colorectal cancer[J]. Biochemical and Biophysical Research Communications, 2018, 497(1): 122~126.
2021, Vol. 27 
冀公网安备13080202000677号