Abstract：Objective: To detect the expressions of microRNA-23b (miR-23b) and notch1 protein in keloid fibroblasts, and to explore the relationship between the expressions and their effects on keloid. Methods: From December 2017 to November 2019, 130 patients with keloid were collected from the 149 Hospital of the PLA. During the operation, the scar tissues and normal dermal tissues of each patient were taken at the same time. The scar size was recorded, According to the severity of keloid, it was divided into mild 65 cases, moderate 43 cases and severe 22 cases. Firstly, fibroblasts from keloid and normal dermis were extracted and cultured, the level of miR-23b in fibroblasts was detected by real-time fluorescent quantitative PCR (qRT-PCR), and the level of notch1 protein was detected by immunohistochemistry. Pearson method was used to evaluate the correlation between miR-23b and notch1 expressions in keloid. Results: There were 203 skin lesions in 130 patients with keloids, 78 on the chest (38.42%), 59 on the shoulder and back (29.07%), 40 on the limbs (19.71%), 13 on the ears (6.40%), and 8 on the abdomen (3.94%), 5 on the hip (2.46%). The cumulative area of skin lesions was 6 cases (4.61%) with large scars, 57 cases (43.85%) with medium scars, and 67 cases (51.54%) with small scars. Compared with normal dermal tissues, mir-23b mRNA expression level and Notch1 positive expression rate in fibroblasts in keloid tissues were significantly increased, with statistically significant differences (66.15% vs 40.00 %, P<0.05). The expressions of miR-23b mRNA and notch1 in different clinical grades of keloid were statistically significant (P<0.05), and with the aggravation of keloid, miR-23b mRNA level and notch1 positive expression rate increased; Pearson correlation analysis showed that miR-23b and Notch-1 were positively correlated in keloid tissue fibroblasts (r = 0.529, P<0.05). Conclusions: MiR-23b mRNA and Notch-1 protein are highly expressed in keloid tissue, and they are positively correlated, which may be involved in the formation and development of keloid at the same time.
何雪冬, 王娴. miR-23b和notch1在瘢痕疙瘩中的表达关系研究[J]. 河北医学, 2020, 26(12): 2016-2020.
HE Xuedong, et al. Research on the Expression Relationship of MiR-23b and Notch1 in Keloid. HeBei Med, 2020, 26(12): 2016-2020.
[1] Sara U, Ardeshir BM. Keloid scarring or disease: Unresolved quasi-neoplastic tendencies in the human skin[J]. Wound Repair Regen, 2020,28(3):422~426. [2] Diaz A, Tan K, He H, et al. Keloid lesions show increased IL-4/ IL-13 signaling and respond to Th2-targeting dupilumab therapy[J].Eur Acad Dermatol, 2020,34(4):161~164. [3] Shen Y, Pan X, Yang J. Gene regulation and prognostic indicators of lung squamous cell carcinoma: TCGA-derived miRNA/mRNA sequencing and DNA methylation data[J].Cell Physiol, 2019, 234(12):22896~22910. [4] Kurkewich JL, Boucher A, Klopfenstein N, et al. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation[J]. Exp Hematol, 2018, 59(1):14~29. [5] 王艳,费明珠,陈姣姣,等.苦瓜蛋白通过下调miR-23b-3p促进三磷酸腺苷结合盒转运体A1表达[J].中国动脉硬化杂志,2018,26(4):335~341. [6] Han B, Fan J, Liu L, et al. Adipose-derived mesenchymal stem cells treatments for fibroblasts of fibrotic scar via downregulating TGF-β1 and notch-1 expression enhanced by photobiomodulation therapy[J]. Lasers Med Sci, 2019, 34(1):1~10. [7] 方莉莉,安玲,荆海霞,等.孕激素受体在不同发育期牦牛乳腺组织内的分布及表达[J].黑龙江畜牧兽医,2019,8(11):141~146. [8] Fathia MK, Mohamad N, Shrook AK, et al. Combination of pulsed dye laser and verapamil in comparison with verapamil alone in the treatment of keloid[J].Dermatol Treat, 2020,31(2):186~190. [9] Yicel BH, Michelle AVQ, Abril AQB, et al. Surface brachytherapy in the treatment of keloid scars in Mexico[J]. Rep Pract Oncol Radiother, 2020,25(1):133~138. [10] Lee YI, Kim J, Yang CE, et al. Combined therapeutic strategies for keloid treatment[J]. Dermatol Surg, 2019, 45(6):802~810. [11] Felice BD, Manfellotto F, Garbi C, et al. MiR-34 modulates apoptotic gene expression in Ingenol mebutate treated keloid fibroblasts[J]. Mol Med Rep, 2018, 17(5):7081~7088. [12] He RQ, Wu PR, Xiang XL, et al. Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification[J]. Int Mol Med, 2018, 41(5):2813~2831. [13] Lyu L, Zhao Y, Lu H, et al. Integrated Interaction network of MicroRNA target genes in keloid scarring[J]. Mol Diagn Ther, 2019, 23(1):53~63. [14] Backer RA, Hombrink P, Helbig C, et al. The fate choice between effector and memory T cell lineages: asymmetry, signal integration and feedback to create bistability[J]. Adv Immunol, 2018, 137(1):43~82. [15] Guo H, Wang F, Diao Y, et al. Knockdown of Notch1 inhibits nasopharyngeal carcinoma cell growth and metastasis via downregulation of CCL2, CXCL16, and uPA[J]. Mol Carcinogen, 2019, 58(10):1886~1896.
2020, Vol. 26 
冀公网安备13080202000677号