Abstract：Objective: To investigate the expression of connexin 43(Cx43) and p70S6 kinase (p70S6K) in gastric cancer tissues and their correlation with the clinicopathological parameters.Methods: The protein expression of Cx43 and p70S6K in gastric cancer and normal gastric mucosa was detected by Western blot assay.The mRNA expression of Cx43 and p70S6K genes was detected by quantitative real-time PCR.Results: At the protein level,the expression of Cx43 in gastric cancer tissues M(0.39,0.98) was lower than that in normal gastric mucosa tissues M(1.62,3.80),and the expression level of p70S6K M(0.94,1.55) was higher than that in normal gastric mucosa tissues M(0.54,1.02),and the differences were statistically significant (all P<0.01).At the gene level,the positive expression of Cx43 mRNA in gastric cancer tissues M(0.33,0.66)was lower than that in normal gastric mucosa tissues M(1.00,1.00),and the positive expression of p70S6K mRNA in gastric cancer tissues M(2.14,4.14) was significantly higher than that in normal gastric mucosa tissues M(1.00,1.00),and the differences were statistically significant (all P<0.01).No matter at the protein level or gene level,with the worse differentiation of gastric cancer,the higher the TNM stage,the deeper the infiltrating muscle layer,and the lower the positive expression rate of Cx43 in lymph node metastasis;on the contrary,the positive expression rate of p70S6K increased significantly (all P<0.05),regardless of gender and age (all P>0.05).There was a negative correlation between Cx43 and p70S6K protein expression in gastric cancer tissues (r=-0.746,P＜0.01).There was a negative correlation between Cx43 and p70S6K mRNA expression (r=-0.759,P＜0.01).Conclusion: The abnormal expression of Cx43 and p70S6K in gastric cancer may play an important role in the occurrence and development of gastric cancer.
刘莎莎, 刘欢, 赵杨, 申兴斌. 胃癌组织中Cx43与p70S6K的表达及其临床意义[J]. 河北医学, 2020, 26(11): 1810-1814.
LIU Shasha , LIU Huan , ZHAO Yang , et al. Clinical Significance and Expression of Cx43 and p70S6K in Gastric Cancer. HeBei Med, 2020, 26(11): 1810-1814.
[1] S M Wang,R S Zheng,S W Zhang,et al.Epidemiological characteristics of gastric cancer in China,2015[J].Zhong hua liu xing bing xue za zhi,2019,40(12):1517~1521. [2] Jung KW,Won YJ,Kong HJ,et al.Cancer statistics in Korea:incidence,mortality,survival,and prevalence in 2016[J].Cancer Res Treat,2019,51(2):417~430. [3] Xin Lian,Junlian Gu,Baoshan Gao,et al.Fenofibrate inhibits mTOR-P70S6K signaling and simultaneously induces cell death in human prostate cancer cells[J].Biochemical and biophysical research communications,2018,496(1):70~75. [4] Maria Chasampalioti,Andrew R Green,Ian O Ellis,et al.Connexin 43 is an independent predictor of patient outcome in breast cancer patients[J].Breast cancer research and treatment,2019,174(1),93~102. [5] 张立伟,张爱东,谭淑娟,等.胃癌中Cx43与P-Ezrin表达的意义及相关性研究[J].重庆医学,2018,47(17):2353~2355,2358. [6] Jiang Kehua,Chen Hongbo,Tang Kun et al.Puerarin inhibits bladder cancer cell proliferation through the mTOR/p70S6K signaling pathway[J].Oncology Letters,2018,15(1):167~174. [7] Zhou Honghui,Zhao Haibo,Liu Hui,et al.Influence of carboplatin on the proliferation and apoptosis of ovarian cancer cells through mTOR/p70s6k signaling pathway.[J].Journal of B.U.ON.:official journal of the Balkan Union of Oncology,2018,23(6),1732~1738. [8] 陈瑞红,徐刚,李远发,等.胃癌组织GOLPH-3介导mTOR信号通路表达[J].中华肿瘤防治杂志,2019,26(21):1611~1616.
2020, Vol. 26 
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