Abstract：Objective: To investigate the expression level of PI3K/AKT signal pathway in the abdominal aorta of diabetic rats and the intervention mechanism of berberine. Methods: The SD rats were randomly divided into 3 groups, the normal control group, diabetic model group and berberine intervention group, 20 rats in each group. The diabetic rat model was established by intraabdominal injection of streptozotocin. The rats in berberine intervention group received berberine gastric perfusion with the dosage of 100mg·kg-1·d-1 while other 2 groups with the same dosage of normal saline after the model were established successfully. After 6 weeks, the blood glucose and lipid level were compared before and after intervention. The abdominal aorta were taken out and the PI3K, AKT, p21 expression level were detected by immunohistochemical technique. Results: The blood glucose level of diabetic model rats increased after modeling. The blood glucose level of the berberine intervention group decreased after pharmacological intervention and lower than that of the diabetic model group. There was no statistically significant difference of TC and TG between 3 groups before and after intervention. The expression level of PI3K, AKT in abdominal aorta were significant high while the level of p21 was low in the diabetic model group. BBR could decrease the level of blood glucose and inhibit the expression of PI3K, AKT while upregulating the level of p21 in abdominal aorta of diabetic rats. Conclusion: The activation of PI3K/AKT signal pathway may be one of the pathogenesis of DA. BBR may intervene the pathogenesis of DA by inhibiting cell proliferation by regulating the PI3K/AKT signal pathway.
朱昌国, 李帅, 孙艳君, 王玉涛, 孙岩. 小檗碱调控PI3K/AKT信号通路干预糖尿病动脉硬化的实验研究[J]. 河北医学, 2020, 26(12): 1937-1942.
ZHU Changguo, LI Shuai, SUN Yanjun, et al. The Experimental Research of Berberine Intervening Diabetic Atherosclerosis by Regulating PI3K/AKT Signal Pathway. HeBei Med, 2020, 26(12): 1937-1942.
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2020, Vol. 26 
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