Abstract：Objective: To study the expression and clinical significance of cyclin B1 (CCNB1) and ubiquitin binding enzyme E2S (UBE2S) in glioma.Methods: 106 cases of glioma tissue specimens from January 2015 to April 2019 were selected and brain glial tissue specimens from 114 patients with intracranial hemorrhage or trauma undergoing intracranial decompression were taken as control group.The expression levels of CCNB1 and UBE2S in glial tissue were measured by immunoblotting (WB).The relationships between the expression levels and clinicopathological features of glioma were analyzed.All patients were followed up.The effects of the expressions of both on the prognosis of patients with glioma were explored.Results: The expression levels of CCNB1 and UBE2S in low-grade glioma group and high-grade glioma group were significantly higher than those in the control group (P<0.05),and the expression levels of CCNB1 and UBE2S in high-grade glioma group was significantly higher than that in low-grade glioma group (P<0.05).The expression levels of CCNB1 and UBE2S in glioma tissues were not correlated with age,sex and pathological type (P>0.05),but were related to the degree of tissue differentiation,peritumoral edema and infiltration (P<0.05).Pearson analysis showed that the expression levels of CCNB1 and UBE2S in glioma tissues were positively correlated (r = 0.460,P<0.05).The median survival time of patients with high expressions of CCNB1 and UBE2S were significantly lower than those with low expressions of CCNB1 and UBE2S (P<0.05).COX multivariate analysis showed that low tissue differentiation,high expression of CCNB1 and UBE2S were independent risk factors for poor prognosis of glioma patients (P<0.05).Conclusion: The expression levels of CCNB1 and UBE2S in glioma tissues are significantly increased,which is related to the degree of differentiation,peritumoral edema,infiltration and survival time of glioma tissues,and might be a clinical index for evaluating the prognosis of glioma patients.
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2020, Vol. 26 
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