Abstract：Objective: To study the effects of anti-bronchial syrup combined with glucocorticoid on NF-κB signaling pathway in model rats with cough variant asthma. Methods: 50 SD rats were selected and randomly divided into control group, model group, anti-bronchial syrup group, glucocorticoid group and combined group, with 10 rats in each group. On the 1st day of the experiment, the other four groups except control group were injected intraperitoneally with ovalbumin (OVA) and aluminum hydroxide, and they were treated for three times with once/7days for rat asthma model construction. On the 21st day of the experiment, they were given ovalbumin inhalation for atomization stimulation, and control group was stimulated with normal saline for 3 times/week with 30 min/time. Each drug-administered group was pretreated with drugs at 30 min before atomization stimulation, and control group was treated with the same amount of normal saline. 3 weeks after stimulation, HE staining was used to observe the pathological changes of the lung tissue, and the thickness of airway wall and the thickness of smooth muscle were measured. Western blot was used to detect the expression levels of IκB, NF-κB, MMP-9 and VEGF proteins in rat lung tissue. Results: HE staining showed that combined group could significantly improve the airway wall epithelial tissue necrosis and shedding, and a large number of inflammatory cell infiltration. The analysis of airway wall and smooth muscle thickening showed that the airway wall and smooth muscle thickening in the other four groups were significantly thickened compared with control group (P<0.05). Compared with model group, the degree of tissue thickening in drug-treated groups was significantly decreased, and the degree in combined group was significantly lower than that in single drug groups (P<0.05). Compared with control group, the expression level of IκB protein was significantly decreased in the other four groups, and the expression levels of NF-KB, MMP-9 and VEGF were significantly increased (P<0.05). Compared with model group, the expression level of IKB protein in single drug groups and combined group was significantly increased, and the expression levels of NF-KB, MMP-9 and VEGF were significantly decreased (P<0.05), and the changes of protein expression in combined group were significantly higher than those in single drug groups (P<0.05). Conclusion: Kangzhi syrup combined with dexamethasone can reduce the hydrolysis of I κ B protein, decrease the release of NF-κ B protein, down regulate the expression of MMP-9 and VEGF, and inhibit the airway remodeling of cough variant asthma rats.
[1] 楚慧伦,孔德明,丁子桐,等.Brown-Norway大鼠咳嗽变异性哮喘模型的建立[J].中国比较医学杂志,2018,28(3):63~66. [2] CHEN M, LV Z, ZHANG W, et al. Triptolide suppresses airway goblet cell hyperplasia and Muc5ac expression via NF-κB in a murine model of asthma[J]. Mol Immunol,2015,64( 1) : 99~105. [3] 田雪,周新.咳嗽变异性哮喘诊治新进展[J].华西医学,2018,33(1):99~103. [4] 刘嘉研,李俊峰,车楠,等.梓醇对哮喘小鼠气道炎症及AMPK/ROS/NF-κB信号通路的影响[J].郑州大学学报:医学版,2019,54(6):823~827. [5] 童骄,朱文雄,曾逸笛,等.清肝宁肺汤对咳嗽变异性哮喘大鼠模型疗效的实验研究[J].湖南中医药大学学报,2012,32(3):23~26. [6] 邹晖,徐永健,张珍祥,等.血管内皮生长因子及其受体2与哮喘模型大鼠气道平滑肌细胞增殖关系的研究[J].中国组织化学与细胞化学杂志,2010,19(4):321~326. [7] 曲耘,吴荣,张晓晔.NF-κB/p65siRNA对小鼠Lewis肺癌移植瘤细胞凋亡和Bax表达的影响[J].中国医科大学学报,2017,46(2):145~148. [8] Taniguchi K, Karin M. NF-κB inflammation immunity and cancer: coming of age[J]. Nature Reviews Immunology, 2018, 18(5):309~324. [9] Cheng CC,Guan SS,Yang HJ, et al.Blocking hemeoxygenase-1 by zinc protoporphyrin reduces tumor hypoxia-mediated VEGF release and inhibits tumor angiogenesis as a potential therapeutic agent against colorectal cancer[J].Biomed Sci,2016,23(1):18~25.
2020, Vol. 26 
冀公网安备13080202000677号