Beclin1调控的自噬在胚胎碎片形成中的作用及机制研究

doi:10.3969/j.issn.1006-6233.2020.10.004

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摘要 目的: Beclin1调控的自噬在胚胎碎片形成中的作用及可能机制。方法: 选择2016年7月至2019年10月我院行取卵体外受精的患者240例,选取胚胎(3PN)后根据碎片含量不同,细分为如下四组:低碎片组(碎片比例<10%)、中低碎片组(25%>碎片比例≥10%)、中高碎片组(50%>碎片比例≥25%)、高碎片组(碎片比例≥50%),每组均60例。使用G2囊胚培养液对所选胚胎进行培养,使用干扰素(浓度40ng/mL)干扰36h。采用免疫印迹法定量检测Beclin1蛋白、PI3K蛋白、LC3B蛋白、mTOR蛋白、AKT蛋白含量。结果: 干扰前后比较,四组患者Beclin1蛋白、PI3K蛋白、LC3B蛋白、mTOR蛋白、AKT蛋白含量均显著增加,差异均有统计学意义(P<0.05)。干扰前比较6项蛋白含量,从高到低依次为:低碎片组、中低碎片组、中高碎片组、高碎片组,差异均有统计学意义(P<0.05)。干扰后比较,四组患者Beclin1蛋白、PI3K蛋白、LC3B蛋白含量的差异均有统计学意义(P<0.05)。Beclin1与PI3K、LC3B、mTOR、AKT相关性均有统计学意义(P<0.05)。结论: 高比例胚胎碎片将抑制Beclin1、PI3K、LC3B、mTOR、AKT蛋白含量,Beclin1调控自噬过程可能产生干扰素类似效应,从而影响相关通道蛋白的表达。

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关键词 ： Beclin1, 自噬, 胚胎碎片, 通道蛋白

Abstract：Objective: To study the role and possible mechanism of beclin1-regulated autophagy in embryo fragment formation. Methods: A total of 240 patients who underwent in vitro fertilization of eggs in our hospital from July 2016 to October 2019 were selected. Embryo (3PN) was selected and subdivided into the following four groups according to different fragment contents: low fragment group (fragment proportion <10%), medium and low fragment group (25%> fragment proportion ≥10%), medium and high fragment group (50%> fragment proportion ≥25%), and high fragment group (fragment proportion ≥50%), with 60 cases in each group. The selected embryos were cultured in G2 blastocyst medium and interferon (40ng/mL) interferon was used for 36h. Beclin1, PI3K, LC3B, mTOR and AKT were quantitatively determined by western blot. Results: Before and after interference, Beclin1 protein, PI3K protein, LC3B protein, mTOR protein and AKT protein content in the four groups were significantly increased, with statistically significant differences (P<0.05). Before interference, the contents of the six proteins were compared, from high to low: low fragment group, medium and low fragment group, medium and high fragment group, and high fragment group, with statistically significant differences (P<0.05). After infection, the contents of Beclin1 protein, PI3K protein and LC3B protein in the four groups were significantly different (P<0.05). Beclin1 was significantly correlated with PI3K, LC3B, mTOR and AKT (P<0.05). Conclusion: A high proportion of embryo fragments will inhibit Beclin1, PI3K, LC3B, mTOR and AKT protein content. Beclin1's regulation of autophagy may produce interferon-like effects, thus affecting the expression of related channel proteins.

Key words： Beclin1 Autophagy Embryo fragments Channel protein

基金资助:山东省自然科学基金,(编号:ZR2017LH011)

通讯作者: 金真真

引用本文:

牛焕付, 牛姝方, 魏泽锋, 于春娜, 金真真. Beclin1调控的自噬在胚胎碎片形成中的作用及机制研究[J]. 河北医学, 2020, 26(10): 1600-1604.
NIU Huanfu, NIU Shufang, WEI Zefeng, et al. Study on the Role and Mechanism of Beclin1-Regulated Autophagy in Embryo Fragment Formation. HeBei Med, 2020, 26(10): 1600-1604.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2020.10.004 或 http://www.hbyxzzs.cn/CN/Y2020/V26/I10/1600

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