Abstract：Objective: To investigate the effects of three kinds of materials, namely, homologous (human skin) acellular matrix, heterogeneous (cowhide) acellular matrix, and nanocellulose protein in the repair of oral mucosa defects in rats. Methods: 80 3-month-old male Wister clean rats were randomly divided into three groups: group A (nanocellulose silk fibroin group, n=20), group B (cowhide acellular matrix group, n=20), group C (human skin acellular matrix group, n=20), and group D (blank control group, n=20). Full-thickness buccal mucosa with a diameter of 10 mm was excised from rats in each group. Wounds of rats in groups A, B, and C were respectively covered with silk fibroin, heterogeneous (cowhide) acellular matrix, and homologous (human skin) acellular matrix which had the same diameter and were treated with radicidation. The wounds of rats in group D were covered with vaseline gauze. The wound areas were compared among groups at 1 week, 2 weeks, 3 weeks and 4 weeks after surgery. The numbers of fibroblasts, neovascular endothelial cells and inflammatory cells, and growth of endothelial cells in rats at different time points were analyzed through HE staining and immunohistochemistry. Results: ①There was no wound infection in each group. Transparent membranes could be observed in the grafted area of group A, B, and C at 1 week after surgery. The wound margin of group D was slightly red and swollen. The wound diameter of group D was significantly larger than that of group A, B, and C at 1 week, 2 weeks, and 3 weeks after surgery (P<0.05), without significant difference in wound size among group A, B, and C at each time point (P>0.05). ② HE staining showed that the numbers of fibroblasts and inflammatory cells in group A, B, and C at different time points were significantly smaller than those in group D (P<0.05), without significant difference among group A, B, and C at each time point (P>0.05). ③Immunohistochemistry showed that the expression of CK-positive cells in group A, B, and C was significantly higher than that in group D at 1 week, 2 weeks, 3 weeks and 4 weeks after surgery (P<0.05). The number of CD34-positive cells was significantly larger than that in group D at 1 week, 2 weeks, and 3 weeks after surgery (P<0.05), without significant difference among group A, B, and C at each time point (P>0.05). Conclusion: The effects of nanocellulose protein, autologous and allogeneic cell matrices are similar in repair of oral mucosa damage in rats.
韩永洁, 刘世杰, 邓露. 三种不同材料修复大鼠口腔黏膜缺损创面的对比实验研究[J]. 河北医学, 2020, 26(7): 1209-1213.
HAN Yongjie, LIU Shijie, DENG Lu. Comparative Experimental Study on Three Different Materials for Repairing Oral Mucosa Defects in Rats. HeBei Med, 2020, 26(7): 1209-1213.
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2020, Vol. 26 
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