Abstract：Objective: To investigate the effect mechanism of miR-23a on the proliferation of human tongue squamous cell carcinoma cells. Methods: Clinical tissue samples of tongue squamous cell carcinoma were collected and Tca8113 and CAL27 were cultured. Real-time quantitative PCR was performed to detect the expressions of miR-23a and PPP2R5E in Clinical tissue samples of tongue squamous cell carcinoma. MTT assay, colony formation assay and growth curve assay were used to detect the effect of miR-23a and PPP2R5E on the proliferation of human tongue squamous carcinoma cell. Luciferase reporter assay verified the regulatory relationship between mir-23a and PPP2R5E. Results: The expression of miR-23a in tongue squamous cell carcinoma was significantly up-regulated(P<0.01). MiR-23a promoted the proliferation of tongue squamous cell carcinoma cells. Bioinformatics prediction and luciferin reporting experiments showed that PPP2R5E was a direct target gene of miR-23a. The expression of PPP2R5E was decreased in tongue squamous cell carcinoma. PPP2R5E inhibited the proliferation of tongue squamous cell carcinoma cells. Overexpression of PPP2R5E can reverse the proliferation promoting effect of mir-23a on tongue squamous cell carcinoma cells. Conclusion: MiR-23a can promote the proliferation of tongue squamous cell carcinoma cells through PPP2R5E. MiR-23a plays an oncogene role in the occurrence and development of tongue squamous cell carcinoma.
[1] Global burden of disease cancer collaboration the global burden of cancer 2013[J].JAMA Oncology, 2015,1( 4) : 505~527. [2] 刘洋,战德松.舌癌动物模型研究进展[J].中国实用口腔科杂志,2013,6(5):311~316. [3] 胡琼丹,胡柯琴,樊均明.miR-216b通过FGFR1调节肾透明细胞癌细胞增殖和侵袭研究[J].重庆医学,2018,47(25):3245~3248. [4] Lanying Sun, et al.MicroRNA-137 suppresses tongue squamous carcinoma cell proliferation, migration and invasion [J].Cell Prolif, 2016,49(5):628~635. [5] Peng F, et al.miR-23a promotes cisplatin chemoresistance and protects against cisplatin-induced apoptosis in tongue squamous cell carcinoma cells through Twist[J]. Oncol Rep, 2015,33(2):942~950. [6] Chen B, et al.miR23a suppresses pancreatic cancer cell progression by inhibiting PLK1 expression [J].Mol Med Rep, 2018,18(1):105~112. [7] Deng Y H, et al.MicroRNA-23a promotes colorectal cancer cell survival by targeting PDK4 [J].Exp Cell Res, 2018,373(1-2):171~179. [8] 盛晶,万里新,屈中玉.miR-23a靶向NID2通过Notch通路调控肺癌细胞的侵袭和迁移[J].肿瘤防治研究,2017,44(6):381~386.
2020, Vol. 26 
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