Abstract：Objective: The theory and experiment of HPLC determination of total solubility parameters of Chinese traditional medicine were studied. Methods: By high performance liquid chromatograph to caffeine, aloe emodin, rhein, emodin and emodin and methyl ether in the liquid phase detection, through the theory of solubility parameter and Hildebrand -Scatchard solution related equation and principle of high performance liquid chromatography (HPLC) combined with each other, treat total component parameter measurement AT, BT, CT and the related fingerprint parameters average retention time analysis the relationship between a, b, c and so on. Results: According to the fingerprint between apparent solubility parameter average retention time and amount of related function and a mathematical model, based on high performance liquid chromatographic peak time correlation formula (1) and (2) different elution conditions of caffeine solubility parameter values were 32.04 J1/2/cm3/2, 35.07 J1/2/cm3/2, 39.04 J1/2/cm3/2, 43.26 J1/2/cm3/2, 45.78 J1/2/cm3/2, 46.22 J1/2/cm3/2. The correlation values calculated by the formula were 0.09256, 0.14365, 27.39, 0.1533 and 0.16743, respectively. According to the fingerprint average retention time and rhubarb components between the solubility parameter of the related function and a mathematical model, based on high performance liquid chromatographic peak time in combination with related formula (1) and (2) different elution conditions of aloe emodin solubility parameter values were 38.56 J1/2/cm3/2、36.54 J1/2/cm3/2、40.25 J1/2/cm3/2、44.25 J1/2/cm3/2、43.65 J1/2/cm3/2、44.58 J1/2/cm3/2. The correlation values calculated by the formula were 1.09584, 0.5869, 26.58, 0.53647 and 0.12356, respectively. Rhubarb acid solubility parameter values were 43.25 J1/2/cm3/2, 44.26 J1/2/cm3/2, 44.58 J1/2/cm3/2, 45.44 J1/2/cm3/2, 44.98 J1/2/cm3/2, 45.23 J1/2/cm3/2, respectively. The solubility parameters of emhutin were 44.73 J1/2/cm3/2、42.16 J1/2/cm3/2、47.69 J1/2/cm3/2、43.21 J1/2/cm3/2、44.78 J1/2/cm3/2、43.26 J1/2/cm3/2, and 0.57657, 0.43256, 0.44569, 0.43225, and 0.44154, respectively. The solubility parameters of emodin methyl ether were 42.16 J1/2/cm3/2、47.56 J1/2/cm3/2、46.21 J1/2/cm3/2、44.38 J1/2/cm3/2、47.56 J1/2/cm3/2、44.23 J1/2/cm3/2, respectively. The relationship between the total parameters AT, BT, CT and the average retention time of the related fingerprint parameters a, b and c was strongly fitted. Through the P value analysis, there were significant differences among the indexes (P<0.01). Therefore, combined with the total solubility parameter theory of related TCM components and high performance liquid chromatography, the average values of fingerprint parameters can be calculated and analyzed. Conclusion: HPLC has a good application value in determining the solubility parameters of multiple components of traditional Chinese medicine and can lay a theoretical foundation for improving the application of single traditional Chinese medicine.
徐程. HPLC测定中药多成分总量溶度参数的实验研究[J]. 河北医学, 2019, 25(10): 1729-1733.
XU Cheng. Experimental Study on Determination of Total Solubility Parameters of Chinese Traditional Medicine by High Performance Liquid Chromatography. HeBei Med, 2019, 25(10): 1729-1733.
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2019, Vol. 25 
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