Abstract：Objective: To analyze the expressions of serum miR-33a and miR-133a in patients with chronic hepatitis B virus (HBV) infection, and to explore their relationships with hepatic fibrosis and their diagnostic values for severe hepatic fibrosis. Methods: From January 2017 to June 2018, 132 patients with chronic HBV infection (hepatitis B group) was conducted liver biopsy, liver fibrosis was graded according to the Metavir system score, including 78 cases of mild to moderate liver fibrosis group and 54 cases of severe liver fibrosis group. At the same time, 100 healthy people without family history of hepatitis B were selected as control group. RT-PCR was used to detect the expression levels of miR-33a and miR-133a in the subjects, Pearson correlation coefficient (r) method and receiver operating characteristic curve (ROC) were used to analyze the relationships between the levels of miR-33a and miR-133a with the grading of hepatic fibrosis in patients with severe hepatic fibrosis, and its diagnostic value for severe liver fibrosis. Results: The expression level of ALT、AST、TBIL、miR-33a in hepatitis B group was higher than that in the control group (P<0.05), and the expression level of miR-133a was lower than that in the control group (P<0.05). The expression level of serum miR-33a in severe liver fibrosis group was higher than that in mild to moderate liver fiber. The level of miR-133a was lower than that of mild to moderate liver fibrosis group (P<0.05); there was a positive correlation between serum miR-33a with liver fibrosis grading in hepatitis B group (r=0.613, P =0.005), there was a negative correlation between miR-133a and liver fibrosis grade (r=-0.598, P =0.009); the area under the ROC curve (AUC) of serum miR-33a and miR-133a in diagnosing severe hepatic fibrosis was 0.740 and 0.694 respectively, the diagnostic critical value of miR-33a was 1.79, the sensitivity and specificity was 74.97% and 89.39% respectively, the diagnostic critical value of miR-133a was 0.86, the sensitivity and specificity was 70.70% and 80.45% respectively. Conclusion: Serum miR-33a levels in patients with chronic HBV infection is increased, the level of miR-133a is decreased. It is related to the grading of liver fibrosis and it can be used as a biomarker for clinical assessment of liver fibrosis.
王慧, 许剑兰, 郑成芳, 陈灵芝. miR-33a miR-133a对慢性乙肝感染患者肝纤维化评估作用[J]. 河北医学, 2019, 25(5): 722-725.
WANG Hui, XU Jianlan, ZHENG Chengfang, et al. The Roles of MiR-33a and MiR-133a in the Assessment of Liver Fibrosis in Patients with Chronic Hepatitis B Infection. HeBei Med, 2019, 25(5): 722-725.
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2019, Vol. 25 
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